妊娠期肝内胆汁淤积症胎盘组织中Caspase-3和Livin表达的相关性研究

目的探讨妊娠期肝内胆汁淤积症（ICP）胎盘中凋亡调节基因Caspase-3和Livin表达的关系。方法以30例ICP患者（ICP组）及21例正常产妇（对照组）为研究对象，应用免疫组化方法测定胎盘凋亡调节基因Caspase-3和Livin的表达水平。结果①ICP组胎盘组织中细胞滋养细胞、合体滋养细胞、蜕膜细胞及间质细胞Caspase-3表达分别为（32．86±6．02）％、（21．57±7．52）％、（27．29±4．89）％、（18．14±3．58）％，明显高于对照组的（14．58±3．41）％、（9．58±2．26）％、（15．00±5．39）％、（9．17±2．20）％。两组比较，差异有显著性（P〈0．05）。②ICP组胎盘组织中细胞滋养细胞、合体滋养细胞、蜕膜细胞及间质细胞中Livin的表达分别为（7．43±2．17）％、（4．71±1．81）％、（4．57±1．21）％、（6．71±2．73）％，明显低于对照组的（14．38±3．24）％、（10．00±3．66）％、（8．75±2．97）％、（12．29±3．59）％。两组比较，差异有显著性（P〈0．05）。③ICP组胎盘组织中合体滋养细胞、细胞滋养细胞、蜕膜细胞及间质细胞Caspase-3和Livin的表达均呈负相关性，相关系数分别为：-0．61、-0．58、-0．57、-0．45，P值均小于0．05。结论ICP组胎盘组织中舍体滋养细胞、细胞滋养细胞、蜕膜细胞及间质细胞Caspase-3表达增高，Livin的表达降低，各细胞中Caspase-3和Livin的表达呈负相关性。ICP组胎盘功能减退与细胞凋亡密切相关，Caspase-3和Livin基因可能是ICP胎盘细胞凋亡的重要调控基因之一。

Study of the correlation between the expressions of caspase-3 and livin in the placenta of patients with intrahepatic cholestasis of pregnancy

Objective To investigate the relationship between the expressions of caspase - 3 and livin in placenta of patients with intrahepatic cholestasis of pregnancy （ICP）. Methods Samples were obtained from 30 cases of ICP （ ICP group） and 21 normal pregnant women （ control group）. The expressions of caspase -3 and livin in placenta tissues were detected by immunohistochemistry method. Results ①The expressions of caspase 3 in placental cytotrophoblast, syncytiotrophoblast, decidual cells and interstitial cells in ICP group were 32.86 ± 6.02 %, 21. 57 ±7.52%, 27.29 ±4.89% and 18.14 ±3.58%, respectively, which were significantly higher than those （ 14.58 ±3.41%, 9.58 ±2.26%, 15. 00 ± 5.39% and 9.17 ± 2.20% ） in control group （ P 〈 0.05）. ②The expressions of livin were 7.43 ± 2.17%, 4.71 ± 1.81%, 4.57 ± 1. 21% and 6.71 ± 2.73%, respectively, which were significantly lower than those （ 14.38 ± 3.24%, 10. 00 ± 3.66%, 8.75 ± 2.97% and 12.29 ± 3.59 % ） in control group （ P 〈 0.05 ）. ③There was a significantly negative correlation between the expressions of caspase - 3 and livin in placental syncytitrophoblast, cytotrophoblast, decidual cells and interstitial cells in ICP group, （ r = -0.61, -0.58, -0.57 and -0.45 respectively, P 〈 0.05 ）. Conclusion ① The expression of caspase - 3 is high, while the expression of livin is low; and there is a negative eorrelation between the expressions of easpase - 3 and livin in placental syncytitrophoblast, cytotrophoblast, decidual cells and interstitial cells in ICP placenta. ②The dysfunction of placenta may closely relate to the apoptosis, and the regulation of caspase - 3 and livin expressions in placenta of patients with ICP may be one of the main reasons for placental apoptosis.