纤维连接蛋白对磷酸钙陶瓷表达修饰促进大鼠成骨细胞的贴附

目的研究大鼠成骨细胞在经纤维连接蛋白表面修饰后的有孔磷酸钙陶瓷上的贴附及生长。方法4块立方形（5mm×5mm×5mm）的有孔磷酸钙陶瓷，随机分成实验组和对照组。实验组陶瓷在负压条件下用重组人纤维连接蛋白(100μg/ml)浸渍过夜。再将两组陶瓷与大鼠成骨细胞共同培养10d，扫描电镜下观察比较实验组与对照组成骨细胞在陶瓷表面的贴附数量和形态。结果实验组成骨细胞的贴附数量显著高于对照组（P<0.01），并分泌大量胶原纤维和形成钙化结节。结论纤维连接蛋白对磷酸钙陶瓷的表面修饰能明显提高大鼠成骨细胞的贴附率，有利于成骨细胞的生长及成骨表型。

Surface modification of calcium phosphate bioceramics by fibronectin does have positive effects on osteoblasts anchorage

AIM: To investigate the effects of fibronectin surface modification on porous calcium phosphate bioceramics to osteoblasts adhesion METHOD: Four cuboid porous ceramics（5mm×5mm×5mm）were divided into experimental and control groups randomly. The experimental group was soaked in 100μg/ml recombinant human fibronectin under negative pressure over night. Then both groups were cultivated in vitro simultaneously with osteoblasts derived from bone marrow stem cells of rats for ten days. The morphogenetic pattern and number of the anchored cells were observed under scanning electron microscope. RESULT: There was statistically significant difference of cell numbers between the experimental and the control groups (P<0.01). Many secretory collagen fiber bundles and mineralized nods were observed in the experimental group under high magnifier. CONCLUSION: It seems that the fibronectin surface coating is beneficial to osteoblast anchorage and its osteogenic phenotype.