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| 高原肺水肿动物模型的初步建立 | |
| 引用本文： | 张兆瑞,张波,何萍萍,王东. 高原肺水肿动物模型的初步建立[J]. 中国医药导报, 2011, 8(27): 27-28,66 |
| 作者姓名： | 张兆瑞 张波 何萍萍 王东 |
| 作者单位： | 空军总医院呼吸内科,北京,100142 |
| 摘 要： | 目的：通过模拟高原低压低氧环境,建立高原肺水肿大鼠动物模型,探讨低氧时间对大鼠肺水肿程度影响。方法：雄性Wistar大鼠随机分为3组,分别为对照组、24 h低氧组、48 h低氧组。比较大鼠肺湿干比、肺组织匀浆中一氧化氮合酶（NOS）、内皮素-1（ET-1）及肺组织病理形态学的变化。结果：与地面对照组比较,24 h低氧组与48 h低氧组大鼠肺湿干比及肺组织匀浆内ET-1升高、NOS降低,差异均有统计学意义（均P〈0.05）;病理学显示,24 h低氧组与48 h低氧组均有肺泡间隔增宽、间质充血等间质性肺水肿表现,与24 h低氧组相比,48 h低氧组肺湿干比、ET-1升高,肺组织匀浆内NOS降低,差异有统计学意义（P〈0.05）。结论：低压低氧可以有效诱导大鼠产生间质性肺水肿,且48 h低氧大鼠肺水肿程度较24 h低氧更严重,更适合应于现实研究。 |
| 关 键 词： | 高原病 肺水肿 大鼠 一氧化氮合酶 内皮素-1 |
The primary estibalishment of rats model of high-altitude pulmonary edema |
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| ZHANG Zhaorui,ZHANG Bo,HE Pingping,WANG Dong. The primary estibalishment of rats model of high-altitude pulmonary edema[J]. China Medical Herald, 2011, 8(27): 27-28,66 | |
| Authors: | ZHANG Zhaorui ZHANG Bo HE Pingping WANG Dong |
| Affiliation: | ZHANG Zhaorui,ZHANG Bo*,HE Pingping,WANG Dong Department of Respiratory Physicians,Airforce General Hospital,Beijing 100142,China |
| Abstract: | Objective: To estibalish the rats model of high-altitude pulmonary edema by putting Wistar rats in hypobaric chamber,and compare the severity of pulmonary edema in different hypoxia time.Methods: Male Wistar rats were randomly divided into three groups: control group,24 h hypoxia group,48 h hypoxia group.Except for the control group,rats in the other groups were put in hypobaric chamber for 24 h and 48 h to produce a rat model of high-altitude pulmonary edema(HAPE).The wet to dry ratio(W/D),lung oxide synth... |
| Keywords: | High altitute sickness Pulmonary edema Rats Nitrix oxide synthase Endothelin-1 |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |