| NDRG2对乳腺癌细胞增殖的影响 |
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| 引用本文： | 朱财林,李南林,王廷,王岭. NDRG2对乳腺癌细胞增殖的影响[J]. 医学争鸣, 2009, 30(11): 961-963 |
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| 作者姓名： | 朱财林 李南林 王廷 王岭 |
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| 作者单位： | 第四军医大学西京医院血管内分泌外科,陕西,西安,710033;第四军医大学西京医院血管内分泌外科,陕西,西安,710033;第四军医大学西京医院血管内分泌外科,陕西,西安,710033;第四军医大学西京医院血管内分泌外科,陕西,西安,710033 |
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| 基金项目： | 国家高技术研究发展计划(863计划)，陕西省自然科学基金 |
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| 摘 要： | 目的：观察NDRG2（N—myc downstream regulated gene 2）对于乳腺癌细胞系增殖的影响．方法：采用脂质体转染法将NDRG2基因转入乳腺癌细胞系SK-BR-3，经G418筛选后获得稳定高表达NDRG2的亚克隆细胞系SK—BR-3／NDRG2-flag及仅转染载体的SK—BR-3／pcDNA3．1（＋）．应用Western Blot检测NDRG2在转染细胞中的表达；应用平板克隆形成试验、MTT、流式细胞仪检测转染前后细胞增殖能力的差异．结果：成功建立了稳定高表达NDRG2的亚克隆细胞系SK—BR-3／NDRG2-flag；NDRG2的高表达导致了乳腺癌细胞系增殖能力减弱（P〈0．01）：细朐周期阳滞存G0／G1期．结论：存乳腺痛细胞系SK—BR-3中，过表达NDRG2可有效抑制细胞增殖并导致细胞周期的阻滞．
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| 关 键 词： | NDRG2 基因 肿瘤 转染 |
Effect of NDRG2 on proliferation of breast cancer cells |
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| ZHU Cai-Lin,LI Nan-Lin,WANG Ting,WANG Ling. Effect of NDRG2 on proliferation of breast cancer cells[J]. Negative, 2009, 30(11): 961-963 |
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| Authors: | ZHU Cai-Lin LI Nan-Lin WANG Ting WANG Ling |
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| Affiliation: | ZHU Cai-Lin,LI Nan-Lin,WANG Ting,WANG Ling Department of Vascalar and Endocrine Surgery,Xijing Hospital,Fourth Military Medical University,Xi'an 710033,China |
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| Abstract: | AIM:To explore the effect of gene NDRG2(N-myc downstream regulated gene 2) transfection on the proliferation and apoptosis in breast cancer cells.METHODS:pcDNA3.1-NDRG2-flag and eukaryotic expression vectors pcDNA3.1(+) were transfected into breast cancer cell line SK-BR-3 by induction of liposome.The subclone cell lines SK-BR-3/NDRG2-flag expre-ssing NDRG2 stably and highly were obtained by persistent G418 selection.NDRG2 expression levels in the transfected cell lines were detected by Western blot and the... |
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| Keywords: | NDRG2 neoplasms transfection |
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