新发展晚期血吸虫病患者血清肽谱分析

目的:分析新发展晚期血吸虫病患者血清多肽,筛选有潜在应用价值的标志蛋白,为其诊断与发病机制研究奠定基础?方法:采集新发展晚期血吸虫病患者与健康人血清,利用包被有弱阳离子的磁珠富集纯化血清多肽,应用基质辅助激光解析电离时间飞行质谱(MALDI-TOF-MS)Flex分析技术结合Mascot生物信息学分析血清多肽指纹图谱?结果:与健康对照组比较,在0.8~13.0 ku区段,新发展晚期血吸虫病患者血清中m/z 2 082?4 282多肽表达上调(P < 0.01),2 661?2 991?3 241?5 337?5 906多肽表达下调(P < 0.05);常规晚期血吸虫病患者血清中m/z 2 662?4 209?5 337与5 906处显著下调,2 082处显著上调?结论:m/z 3 241?4 282血清差异多肽对新发展晚期血吸虫病患者的诊断可能具有潜在应用价值,对该病发生发展的机制也奠定了理论基础?

Peptides analysis from sera of newly-developed advanced schistosomiasis patients

Objective:To screen the peptides biomarker from sera of the newly-developed advanced schistosomiasis (NDAS) patients and to lay the foundation for schistosomiasis diagnosis and pathogenesis. Methods:Peptides were collected from the sera of NDAS patients and health control by MB-WCX kit (Bruker Daltonics GmBH),followed by analysis with Flex-Analysis,ClinProTool algorithm and MASCOT using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. Results:In the mass range from 0.8 to 13 ku,7 peaks with a clear difference in amplitude between the NDAS patients and control groups were detected,5 peaks with mass charge ratio (m/z) of 2 661,2 991,3 241,5 337 and 5 906 were down-regulated and 2 peaks with a m/z of 2 082,4 282 were up-regulated in NDAS patients. Furthermore,m/z of 2 662,4 209,5 337,5 906 were down-regulated and 2 082 was up-regulated in advanced schistosomiasis patients. Conclusion:m/z 3 241,4 282 could give a potential application value for NDAS patients diagnosis and lay the foundation for schistosomiasis pathogenesis.