| ATP敏感性钾通道P266T突变对离子通道特性的影响 |
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| 引用本文： | 万军,吴钢,黄从新,蒋学俊,李丽. ATP敏感性钾通道P266T突变对离子通道特性的影响[J]. 中国病理生理杂志, 2007, 23(1): 18-21. DOI: ISSN:1000-4718 |
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| 作者姓名： | 万军 吴钢 黄从新 蒋学俊 李丽 |
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| 作者单位： | 1武汉大学人民医院心内科, 湖北 武汉 430060; 2 Department of Biology, Georgia State University, 24 Peachtree Center Avenue Atlanta, Georgia |
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| 基金项目： | 国家自然科学基金;湖北省卫生厅青年科技人才基金 |
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| 摘 要： | 目的： 通过通道蛋白特定位点(P266T)突变,观察对ATP敏感性钾通道电生理特性的影响。 方法: 将Kir6.2及其突变子P266T的cDNA导入人胚肾细胞，表达ATP敏感性钾通道，用膜片钳方法研究KATP电生理特性。 结果: KATP(P266T)开放能力是野生型的2倍; KATP(P266T)密度仅是野生型20%; KATP(P266T)对ATP敏感性降低;对pH敏感性增高。 结论: KATP特定位点(P266T)突变可改变KATP电生理特性。
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| 关 键 词： | 钾通道 ATP敏感 突变 电生理学 |
| 文章编号： | 1000-4718（2007）01-0018-04 |
| 收稿时间： | 2005-04-12 |
| 修稿时间： | 2005-04-122006-03-28 |
Effects of P266T mutation on the characteristics of ATP-sensitive potassium channel |
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| WAN Jun,WU Gang,HUANG Cong-xin,JIANG Xue-jun,LI Li. Effects of P266T mutation on the characteristics of ATP-sensitive potassium channel[J]. Chinese Journal of Pathophysiology, 2007, 23(1): 18-21. DOI: ISSN:1000-4718 |
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| Authors: | WAN Jun WU Gang HUANG Cong-xin JIANG Xue-jun LI Li |
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| Affiliation: | 1 Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan 430060, China,E-mail:circulation@sohu.com; 2 Department of Biology, Georgia State University, 24 Peachtree Center Avenue Atlanta, Georgia |
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| Abstract: | AIM:The activity of ATP-sensitive potassium ion channel(K_ ATP)is related to the myocardial function and its electrophysiology.In this study,we investigated the effect of the modulation of ATP-sensitive potassium channel on the electrophysiology of ATP-sensitive potassium channel.METHODS:cDNA of Kir6.2 and its mutation type P266T were transfected into the embryonic kidney cells to express K_ ATP.The electrophysiology of K_ ATP was investigated by using the whole-cell patch-clamp method.RESULTS:The open-state probability of P266T was twice as high as the wild-type channel,while its channel density was only 20% of the wild-type.P266T mutation significantly reduced the ATP sensitivity and the pH sensitivity.CONCLUSION:Mutation of P266T changes the electrophysiological characteristics of K_ ATP. |
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| Keywords: | Potassium channels ATP sensitive Mutation Electrophysiology |
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