Determination of polyamines and metabolites in cerebrospinal fluid by isotope dilution mass fragmentography, and a clinical application |
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| Authors: | G A van den Berg J M Schaaf G T Nagel A W Teelken F A Muskiet |
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| Affiliation: | Central Laboratory for Clinical Chemistry, University Hospital, Groningen, The Netherlands. |
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| Abstract: | Capillary gas chromatography and mass fragmentography was used to determine simultaneously 1,3-diaminopropane, putrescine, cadaverine, spermidine, spermine, isoputreanine and putreanine in cerebrospinal fluid. After addition of deuterium labelled analogs and acid hydrolysis, the compounds were isolated by adsorption onto silica and converted into their N-heptafluorobutyryl-methylesters. Quality control data and an application of the method are given. A patient harbouring an astrocytoma was monitored during chemotherapeutic treatment. |
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