慢性乙型肝炎患者外周循环DNA的定量检测

目的探讨外周循环DNA水平定量检测在诊断肝组织损伤中的价值。方法纳入慢性乙型肝炎轻度、中度、重度患者及健康对照者各30例,采用双重荧光定量PCR法检测血浆中循环DNA的含量。结果健康对照组,慢性乙型肝炎轻度、中度和重度患者外周循环DNA水平分别为(21±9、29±8、38±8和81±16)μg/L,随着慢性乙型肝炎患者病情加重,外周循环DNA水平逐渐升高,慢性乙型肝炎重度患者外周循环DNA水平显著高于健康对照组(P<0.01)。慢性乙型肝炎患者外周循环DNA水平与ALT和AST呈显著正相关(r值分别为0.612和0.632,P值均<0.01)。HBeAg阳性慢性乙型肝炎患者外周循环DNA显著高于HBeAg阴性组(68μg/L±14μg/L vs 23μg/L±12μg/L,P<0.05)。结论外周循环DNA的含量可反映肝脏的炎性反应程度,有可能作为肝脏炎性反应活动程度的一项监测指标。

Quantitative analysis of peripheral circulating DNA level in plasma from patients with chronic hepatitis B

Objective To quantitative analyze peripheral circulating DNA and evaluate its potential clinical value on diagnosis of liver damage.Methods Circulating DNA level of patients with chronic hepatitis B were quantified with a novel duplex real time PCR assay.Results The level of circulating DNA in control group,mild,moderate and severe chronic hepatitis B was（21±9,29±8,38±8 and 81±16）μg/L,respectively.As the condition of patients with chronic hepatitis B exacerbated, circulating DNA level increased gradually.Circulating DNA level of patients with severe chronic hepatitis B was significantly higher than that of control group（P0.01）.There was a positive relationship among circulating DNA level,alanine aminotransferase and aspartate aminotransferase（r =0.612 and 0.632 respectively,P0.01 ）.Circulating DNA level of serum HBeAg positive group was significantly higher than that of serum HBeAg negative group（68μg/L±14μg/L vs 23μg/ L±12μg/L,P0.05）.Conclusion Circulating DNA level may reflect liver inflammation degree and may become a marker of liver inflammation activity.