胃黏膜病变演化过程中抗氧化蛋白Peroxiredoxin6的表达及其与幽门螺杆菌感染的关系

目的探讨胃黏膜病变演化过程中抗氧化蛋白Peroxiredoxin 6(Prx6)表达水平变化及其与幽门螺杆菌(H.pylori)感染的关系。方法根据组织形态学将104例临床内镜检查活检标本分为慢性浅表性胃炎(33例)、慢性萎缩性胃炎(25例)、肠上皮化生(32例)及异型增生(14例)4组。用免疫组织化学方法检测组织标本中Prx6的表达水平。用快速尿素酶试验及Warthin-Starry银染检测H.pylori感染。结果 Prx6在慢性浅表性胃炎、慢性萎缩性胃炎、肠上皮化生、异型增生组的阳性表达率分别为39.4%(13/33)、80.0%(20/25)、93.8%(30/32)、92.9%(13/14),过表达率分别为15.2%(5/33)、44.0%(11/25)、81.3%(26/32)、85.7%(12/14)。慢性浅表性胃炎、慢性萎缩性胃炎组H.pylori阳性者Prx6阳性表达率显著高于H.pylori阴性者(P<0.05),肠上皮化生组Prx6的表达在H.pylori阳性者和阴性者无显著差异(P>0.05)。结论在胃黏膜病变演化过程中,Prx6的表达随着胃黏膜病变的进展而增加,H.pylori在胃黏膜病变演化的早期阶段促进了Prx6的表达。

The relationship between Helicobacter pylori infection and Peroxiredoxin 6 expression in the development of gastric mucosa lesions

Objective To investigate the expression of Peroxiredoxin 6(Prx6) proteins in gastric mucosal lesions and to evaluate the effect of Helicobacter pylori(H.pylori) infection on Prx6 expression in development of gastric mucosal lesions.Methods 104 gastroscopic biopsy samples were divided into four groups of different gastric mucosa lesions according to pathologic findings: chronic superficial gastritis,chronic atrophic gastritis,intestinal metaplasia and dysplasia.Expression of Prx6 was examined by PowerVisionTM immunohistochemistry,and H.pylori status was determined by Warthin-Starry stain and rapid urease test.Results The positive rates of Prx6 in biopsy samples with chronic superficial gastritis,chronic atrophic gastritis,intestinal metaplasia and dysplasia tissues were 39.4%(13/33),80.0 %(20/25),93.8%(30/32) and 92.9%(13/14),and the positive overexpression rates of Prx6 were 15.2%(5/33),44.0%(11/25),81.3%(26/32) and 85.7%(12/14) respectively.Both positive rates of Prx6 expression and overexpression in biopsy samples with H.pylori infection were significantly higher than those without H.pylori infection(P<0.05).The Prx6 expression in chronic superficial gastritis and chronic atrophic gastritis tissues with positive H.pylori was significantly higher than that with negative H.pylori(P<0.05),but it was not observed in intestinal metaplasia and dysplasia tissues.Conclusion Prx6 expression rate in gastric precancerous lesion tissues is increased compared with chronic superficial gastritis.H.pylori infection may also play a role in the early development of gastric precancerous lessions associated with Prx6.