| GA对乳腺癌MDA-MB-435s细胞端粒酶活性的影响 |
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| 引用本文： | 李霞,邓华瑜,陈黎.GA对乳腺癌MDA-MB-435s细胞端粒酶活性的影响[J].重庆医科大学学报,2006,31(5):655-657. |
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| 作者姓名： | 李霞 邓华瑜 陈黎 |
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| 作者单位： | 重庆医科大学基础医学院病理生理教研室,重庆,400016;重庆医科大学基础医学院病理生理教研室,重庆,400016;重庆医科大学基础医学院病理生理教研室,重庆,400016 |
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| 摘 要： | 目的：研究格尔德霉素（geldanamycin，GA）对乳腺癌细胞增殖和端粒酶活性的影响。方法：培养人乳腺癌细胞株MDA—MB-435s，应用MTr法检测GA对MDA—MB-435s细胞的生长抑制作用，流式细胞术分析细胞周期，用TRAP—ELISA法检测细胞端粒酶活性。结果：不同浓度GA对MDA—MB-435s细胞增殖有明显抑制作用，呈时效-量效依赖关系，GA作用后细胞呈现G2／M期阻滞，细胞端粒酶活性下降（P〈O．05）。结论：GA对人乳腺癌MDA—MB-435s细胞增殖具有明显抑制作用，抑制端粒酶活性可能是其发挥抗癌作用的机制之一。
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| 关 键 词： | 乳腺癌 GA 增殖 端粒酶 |
| 文章编号： | 0253-3626(2006)05-0655-03 |
| 收稿时间： | 06 15 2006 12:00AM |
| 修稿时间： | 2006年6月15日 |
Effect of GA on telomerase activity of breast cancer MDA-MB-435s cell |
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| LI Xia,et al.Effect of GA on telomerase activity of breast cancer MDA-MB-435s cell[J].Journal of Chongqing Medical University,2006,31(5):655-657. |
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| Authors: | LI Xia |
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| Institution: | Department of Pathophysiology, College of Basic Medicine, Chongqing Medical University |
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| Abstract: | Objective:To investigate the effect of GA on telomerase activity of breast cancer MDA-MB-435s cell.Methods:Human breast cancer cell line MDA-MB-435s was used in the study.Proliferation of MDA-MB-435s cells was measured with MTT assay.Cell cycle was analyzed by flow cytometry.The telomerase activity was measured by TRAP-ELISA assay.Results:GA inhibited the proliferation of MDA-MB-435s cells on a time-and dose-dependent manner.Aftertreatment of GA,MDA-MB-435s cells arrested at G2 /M phase.The telomerase activity of cells was decreasing compared with that of control cells(P<0.05).Conclusion:GA can prevent breast cancer MDA-MB-435s cells proliferation significantly,which may be related with decreasing the telomerase activity in cells. |
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| Keywords: | GA |
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