| 活化B淋巴细胞与NS_1瘤细胞融合瘤苗的抗肿瘤免疫治疗 |
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| 引用本文： | 赵明耀,董子明,杨洪艳,张义国,郑智敏,吴皓,陈宁,黄幼田,赵勤. 活化B淋巴细胞与NS_1瘤细胞融合瘤苗的抗肿瘤免疫治疗[J]. 郑州大学学报(医学版), 1999, 0(1) |
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| 作者姓名： | 赵明耀 董子明 杨洪艳 张义国 郑智敏 吴皓 陈宁 黄幼田 赵勤 |
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| 作者单位： | 河南医科大学病理生理学教研室，郑州铁路中心医院，河南医科大学生物化学教研室 |
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| 基金项目： | 河南医科大学回国人员科研启动基金,河南医科大学基础医学院科研基金,河南省教委重点攻关项目,河南省科委重点攻关项目 |
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| 摘 要： | 目的：观察活化Ｂ淋巴细胞与ＮＳ１骨髓瘤细胞融合瘤苗对荷ＮＳ１瘤ＢＡＢＬ／Ｃ小鼠免疫治疗作用。方法：用灭活ＮＳ１瘤细胞及其裂解物免疫ＢＡＬＢ／Ｃ小鼠活化的Ｂ淋巴细胞；以５０％ＰＥＧ为融合剂，将免疫脾Ｂ淋巴细胞与野生型ＮＳ１骨髓瘤细胞融合，经ＨＡＴ选择性培养、筛选融合细胞，以融合细胞作为瘤苗，免疫治疗荷ＮＳ１瘤小鼠２次。结果：融合细胞体在宿主体内的致瘤性降低；融合细胞瘤苗免疫治疗荷瘤小鼠的肿瘤组织体积逐渐减小、最终完全消失，小鼠存活期显著延长。结论：活化的Ｂ淋巴细胞与ＮＳ１细胞融合瘤苗能有效诱导小鼠体内的抗肿瘤反应。
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| 关 键 词： | B细胞；NS_1骨髓瘤；细胞融合；肿瘤免疫治疗；小鼠 |
Antitumor immunotherapeutic effects of tumor vaccine prepared by fusion of activated B cells with NS 1 myelema cells |
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| Zhao Mingyao ),Dong Ziming ),Yang Hongyan ),Zhang Yiguo ),Zheng Zhimin ),Wu Hao ),Chen Ning ),Huang Youtian ),Zhao Qin ) ). Antitumor immunotherapeutic effects of tumor vaccine prepared by fusion of activated B cells with NS 1 myelema cells[J]. Journal of Zhengzhou University: Med Sci, 1999, 0(1) |
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| Authors: | Zhao Mingyao ) Dong Ziming ) Yang Hongyan ) Zhang Yiguo ) Zheng Zhimin ) Wu Hao ) Chen Ning ) Huang Youtian ) Zhao Qin ) ) |
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| Affiliation: | Zhao Mingyao 1),Dong Ziming 1),Yang Hongyan 1),Zhang Yiguo 1),Zheng Zhimin 1),Wu Hao 2),Chen Ning 1),Huang Youtian 1),Zhao Qin 3) 1)Department of Pathophysiology,Henan Medical University,Zhengzhou 450052 2) |
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| Abstract: | Aim: To prepare a tumor vaccine by fusion of activated B cells with NS 1myeloma cells and investigate its antitumor immunotherapeutic effects in the NS 1myeloma bearing BALB/C mice. Methods:The activated B cells from the spleen of BALB/C mice immunized with deactivated NS 1myeloma cells and lysates were fused with wild live NS 1myeloma cells by 50% PEG protocol.The resulting fusion cells were selected by HAT medium. The mice loaded NS 1 myeloma cells under their skin were treated with the fused cells twice weekly.Results: The tumorigenicity of the fused cells became decreased apparently,in contrast to that of NS 1cells. The model tumor volumes gradually became small or disappeared at last. The survial time of tumor bearing mice markedly prolonged. Conclusion: The findings of this study indicate the vaccine based on fusion of the acitivated B cells with NS 1 myeloma cells can induce effectively in vivo antitumor immune responses of mice. |
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| Keywords: | B lymphocytes NS 1 myeloma cell fusion tumor immunotherapy mouse |
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