| 高效液相色谱法测定消渴降糖胶囊中槲皮素的含量 |
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| 引用本文： | 赵正保,韩锋,曲婷丽,李晓妮,侯文林. 高效液相色谱法测定消渴降糖胶囊中槲皮素的含量[J]. 中国药物与临床, 2004, 4(12): 919-921 |
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| 作者姓名： | 赵正保 韩锋 曲婷丽 李晓妮 侯文林 |
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| 作者单位： | 1. 山西医科大学药学院药物化学教研室,030001
2. 山西华康药业股份有限公司技术科 |
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| 基金项目： | 山西省运城市产、学、研合作项目 |
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| 摘 要： | 目的建立消渴降糖胶囊中槲皮素含量测定的方法。方法采用高效液相色谱法,色谱柱为Kromasil-C18(4.6mm×250mm,粒径5μm),甲醇-0.2%磷酸溶液(48:52)为流动相,检测波长360nm,流速1.2ml/min,柱温40℃。结果槲皮素在26.24～131.2μg/ml范围内,浓度与峰面积呈线性关系(r=0.9989)。平均加样回收率为100.0%(RSD=3.10%)。结论该方法简单、准确、可靠、专属性强、重现性好,可作为消渴降糖胶囊的质量控制方法。
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| 关 键 词： | 色谱法,高压液相 槲皮素 番石榴叶 消渴降糖胶囊 |
| 修稿时间： | 2004-08-09 |
Quercetin determination by high pressure liquid chromatography in xiaoke jiangtang capsules |
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| ZHAO Zheng-bao,HAN Feng,QU Ting-li,LI Xiao-ni,HOU Wen-lin. Pharmacochemistry Department,College of Pharmacy,Shanxi Medical University,Taiyuan ,China. Quercetin determination by high pressure liquid chromatography in xiaoke jiangtang capsules[J]. Chinese Remedies & Clinics, 2004, 4(12): 919-921 |
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| Authors: | ZHAO Zheng-bao HAN Feng QU Ting-li LI Xiao-ni HOU Wen-lin. Pharmacochemistry Department College of Pharmacy Shanxi Medical University Taiyuan China |
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| Affiliation: | ZHAO Zheng-bao*,HAN Feng,QU Ting-li,LI Xiao-ni,HOU Wen-lin. *Pharmacochemistry Department,College of Pharmacy,Shanxi Medical University,Taiyuan 030001,China |
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| Abstract: | Objective To establish a method to determine the content of quercetin in xiaoke jiangtang capsules. Methods High pressure liquid chromatography (HPLC) was used with Kromasil-C18 column. The mobile phase consisted of methanol-0.2% phosphoric acid (48:52) with a flow rate of 1.2 ml/min, and the wavelength of detector was 360 nm. Results The linear range of quercetin was 26.24～131.2 μg/ml, and the average recovery was 100.0% (RSD=3.10%). Conclusion This method is simple, accurate, reliable with good specificity and reproducibility, it can be used as a quality control method for xiaoke jiangtang capsules. |
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| Keywords: | Chromatography high pressure liquid Quercetin Guava leaf Xiaoke jiangtang capsules |
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