高效液相色谱法测定大籽獐牙菜中有效成分的含量

目的:建立以高效液相色谱法测定大籽獐牙菜中1-羟基-3,7,8-三甲氧基酮、1,5,8-三羟基-3-氧基酮、1,8-二羟基-3,7-二甲氧基)酮和1,8-二羟基-3,5-二甲氧基酮含量的方法。方法:色谱柱为VP-ODSC18(150mm×4.60mm,5μm),流动相为甲醇-水-磷酸(70:30:0.4),检测波长为254nm,流速为1.0mL.min-1,柱温为20℃。结果:1-羟-基3,7,8-三甲氧基酮、1,5,8-三羟基-3-甲氧基酮、1,8-二羟基-3,7-二甲氧基酮和1,8-二羟基-3,5-二甲氧基咄酮进样量分别在0.172～0.86、0.224～1.344、0.144～0.72、0.154～0.77μg范围内线性关系良好,平均回收率(RSD)分别为98.18%(1.19%)、99.32%(1.52%)、98.44%(1.23%)、97.68%(1.57%)。结论:本方法快速、简便,结果准确、可靠。

Determination of the Contents of Effective Constituents in Swertia macrosperma C.B.Clark by HPLC

OBJECTIVE:To establish an HPLC method for the determination of the contents of Decussatin,Bellidifodin,Methylswertianin and Swerchirin in Swertia macrosperma C.B.Clark.METHODS:The determination was performed on VP-ODS C18(150 mm× 4.60 mm,5 μ m),with the mobile phase consisting of methanol-water-phosphoric acid(70∶ 30∶ 0.4),the detection wavelength set at 254 nm,the flow rate at 1.0 mL· min-1 and the column temperature at 20℃.RESULTS:The calibration curves of Decussating,Bellidifodin,Methylswertianin and Swerchirin were in good linearity in the range of 0.172～ 0.86 μ g,0.224～ 1.344 μ g,0.144～ 0.72 μ g and 0.154～ 0.77μ g,respectively.The average recoveries were 98.18%(RSD=1.19%),99.32%(1.52%),98.44%(1.23%)and 97.68%(1.57%),respectively.CONCLUSION:The method is rapid and precise,with accurate and reliable results.