正交试验优选淡竹叶颗粒的闪式提取工艺

目的：采用正交试验优化淡竹叶颗粒的闪式提取工艺。方法以总黄酮、浸膏得率为指标，以正交试验法优化提取次数、乙醇浓度、液料比和提取时间。结果影响淡竹叶颗粒闪式提取效率的因素依次为提取次数＞乙醇浓度＞液料比＞提取时间。最佳提取工艺条件为：采用30倍量80%乙醇，提取3次，3 min/次。工艺验证性试验测得总黄酮得率为29.1 mg/g，浸膏得率为23.6%。结论闪式提取法适合作为淡竹叶颗粒的提取工艺，为淡竹叶颗粒的新工艺开发提供参考。

Optimization of flash type extraction methods forDanzhuye granules by orthogonal test

Objective To optimize the flash type extraction ofDanzhuye Granules.Methods With the total flavonoids and extract yield as indices, the orthogonal test was adopted to optimize the extraction times, the ethanol concentration, solid-liquid ratio, and the duration of extraction forDanzhuye Granules.Results The factors that infuenced the extract efficiency from high to low were the extraction times, volume fraction of ethanol, the ratio of liquid to solid, and the extraction time. The optimum extracting condition included that the origin was extrated by the ethanol volume fraction 80% with the material to liquid ratio 30:1, total of 3 times, with each time of 3 minutes. Validation experiments of the potimal condition showed that the extraction of total flavonoids was 29.1 mg/g, and the extract yield was 23.6%.Conclusions The flash type extraction method is suitable for extraction, which provides a new method for the development and utilization ofDanzhuye Granules.