肺癌外周血VEGF-C及VEGF-R3检测的临床价值

目的 探讨肺癌患者外周血血管内皮生长因子C(VEGF-C)及血管内皮生长因子受体3(VEGF-R3)的表达及其与临床病理特征的关系,并评价其评估淋巴及血行转移的能力.方法 采集124例肺癌患者、30例正常对照外周血,采用酶联免疫吸附试验(ELISA)进行VEGF-C及VEGF-R3的定量检测,分析其与肺癌临床病理特征的关系.结果 肺癌患者血清VEGF-C及VEGF-R3的中位数(四分位数间距)分别为283.57(120.70)pg/ml、62.72(43.02)ng/ml,明显高于正常对照组的234.62(129.20)pg/ml、43.08(17.07)ng/ml(Z=-2.840,P=0.005;Z=-3.834,P<0.001).VEGF-C的表达与患者年龄、性别、原发灶部位及T分期均无关(Z=-0.949,P=0.343;Z=-0.454,P=0.649;Z=-1.168,P=0.243;Z=-1.694,P=0.090),与病理类型、N分期、M分期显著相关(χ2=8.829,P=0.012;χ2=27.148,P<0.001;Z=-2.221,P=0.026).而VEGF-R3的表达与患者年龄、性别、原发灶部位、病理类型及T、N、M分期均无关(Z=-0.558,P=0.577;Z=-0.599,P=0.549;Z=-0.703,P=0.482;χ2=1.166,P=0.558;Z=-0.680,P=0.496;χ2=0.353,P=0.950;Z=-1.523,P=0.128).结论 肺癌患者血清VEGF-C、VEGF-R3的表达水平均高于正常对照组,VEGF-C的表达与患者病理类型、N分期、M分期相关.外周血VEGF-C的检测有望成为辅助评估肺癌淋巴结及远处转移的肿瘤标志物,而VEGF-R3尚未显示出该价值.

Clinical value of the VEGF-C and VEGF-R3 in peripheral blood of patients with lung cancer

Objective To investigate the relationship between the expression of vascular endothelial growth factor C (VEGF-C) and vascular endothelial growth factor receptor-3 (VEGF-R3) in peripheral blood of patients with lung cancer and the pathological characteristics, and to assess the ability to evaluate lymphatic and distant metastasis of the two marks.Methods VEGF-C and VEGF-R3 were detected by enzyme-linked immunosorbent assay (ELISA) in 124 patients with lung cancer and 30 normal controls, and used to analyze the relationship with the pathological characteristics of lung cancer.Results The serum levels [M(QR)] of VEGF-C and VEGF-R3 in patients with lung cancer were 283.57 (120.70) pg/ml and 62.72 (43.02) ng/ml, significantly higher than the control whose VEGF-C and VEGF-R3 were 234.62 (129.20) ng/ml and 43.08 (17.07) pg/ml, respectively (Z=-2.840, P=0.005;Z=-3.834, P<0.001).No correlation was found between the expression of VEGF-C and age, sex, primary tumor site, T stage (Z=-0.949, P=0.343;Z=-0.454, P=0.649;Z=-1.168, P=0.243;Z=-1.694, P=0.090).But the expression of VEGF-C was significantly related with pathologic type, N stage and M stage (χ2=8.829, P=0.012;χ2=27.148, P<0.001;Z=-2.221, P=0.026).However, the expression of VEGF-R3 was not correlated with age, sex, the site of the primary lesion, pathological type and T, N, M stage (Z=-0.558, P=0.577;Z=-0.599, P=0.549;Z=-0.703, P=0.482;χ2=1.166, P=0.558;Z=-0.680, P=0.496;χ2=0.353, P=0.950;Z=-1.523, P=0.128).Conclusion The expressions of VEGF-C and VEGF-R3 in patients with lung cancer are higher than those in normal control, and the expression of VEGF-C is related with patho-logic type, N stage and M stage.The detection of VEGF-C in peripheral blood of lung cancer is expected to be an assistant marker for the evaluation of lymph node metastasis and blood metastasis, but VEGF-R3 does not show its value.