高效液相色谱法测定菝葜散瘀颗粒中黄杞苷和落新妇苷的含量

目的 建立高效液相色谱法同时测定菝葜散瘀颗粒中落新妇苷、黄杞苷的含量.方法 色谱柱为Fortis Cortecstm C18(4.6mm×250mm,5μm),0.05%KH2PO4-乙腈为流动相,梯度洗脱,液相流速1.0ml/min,柱温30℃,检测波长290nm.进样量20μl.结果 落新妇苷、黄杞苷分别在0.0022~0.044μg和0.0124~0.248μg范围内线性关系良好(r=0.9999),2种成分的平均加样回收率为97.0%、97.8%,RSD分别为1.61%,1.65%.结论 本方法灵敏度高、准确、重复性好、专属性强,可作为控制菝葜散瘀颗粒质量的重要方法.

Determination of astilbin and engelitin in Baqia-Sanyu granules

Objective To establish HPLC-DAD method for determination of 2 components in Baqia-sanyu granules.Methods The HPLC system consisted of the Fortis Cortecstm C18 column (4.6 mm× 250 mm, 5 μm), the mobile phase consisted of 0.05%KH2PO4and acetonitrile, gradient elution flow rate was 1.0 ml/min, the column temperature was 30 ℃, and the UV detector was set at 290 nm. Results The linear response range of astilbin and engelitin were 0.002 2-0.044 μg and 0.012 4-0.248 μg(r=0.999 9). The average recovery were 97.0% and 97.8%. RSD were 1.61% and 1.65%. Conclusions The method high sensitive, accurate, repeatable and high specifid. It could be an important method to control the standard of this preparations.