正交试验法优选武当松针茶中总黄酮提取工艺

目的 优选武当松针茶中总黄酮的最佳提取工艺.方法 以总黄酮含量为检测指标,采用L9(34)正交试验考察超声提取中固液比、超声功率、超声时间因素对总黄酮含量的影响.结果 影响提取工艺的因素依次为:固液比>超声功率>超声时间.武当松针茶中总黄酮的最佳提取工艺为:用500倍纯化水,于40℃、500 W功率条件下超声提取15 min.样品中总黄酮的平均含量为39.701 mg/g.结论 优选的提取工艺简单快速,合理可行,用于提取武当松针茶中的总黄酮,为制定其质量标准提供一定基础.

Extraction technology optimization of total flavonoids in Wudang pine needle tea by orthogonal test

Objective To optimize the best extraction technology of total flavonoids in Wudang pine needle tea.Methods The contents of total flavonoids were taken as index, and orthogonal design of L9(34) was applied to process ultrasonic extraction.Results The order of factors of affecting extraction technology was solid to liquid ratio > ultrasonic power > ultrasonic time. The optimized extraction technology was as follows: adding 500 times volume of purified water, ultrasonic extracting for 15 minutes with ultrasound power 500 W at 40℃. The average content of total flavonoids was 39.701 mg/g.Conclusions The optimal extraction technology is simple, efficient and feasible, and can be used for extracting total flavonoids from Wudang pine needle tea, providing basis for the formulation of quality standards.