肉苁蓉多糖对小鼠学习记忆的影响

目的 观察肉苁蓉多糖对不同类型学习记忆障碍模型小鼠空间学习记忆能力的影响,探讨其作用机制.方法 120只小鼠按随机数字表法分为空白组,模型组,肉苁蓉多糖小、中、大剂量组,吡拉西坦组,每组20只.肉苁蓉多糖小、中、大剂量组分别灌胃肉苁蓉多糖溶液25、50、100 mg/kg,吡拉西坦组灌胃吡拉西坦10 mg/kg,空白组和模型组灌胃等体积蒸馏水.1次/d,连续给药6周.分别采用环己米特建立小鼠巩固性记忆障碍模型,采用乙醇建立小鼠再现性记忆障碍模型,采用水迷宫检测各组小鼠学习记忆能力,ELISA法检测各组小鼠脑组织内总蛋白、MDA、SOD水平.结果 与环己米特模型组比较,肉苁蓉多糖中、大剂量组逃避潜伏期(9.45±2.86)s、(12.73±10.89)s比(48.15±30.33)s]、第1次到达站台时间(19.33±3.27)s、(13.81±9.79)s比(40.71±16.76)s]缩短(P<0.05),肉苁蓉多糖小剂量组第1次到达站台时间(11.58±7.04)s比(40.71±16.76)s]缩短(P<0.05),穿越站台次数(5.46±2.09)次比(3.03±1.47)次]增加(P<0.05),肉苁蓉多糖大剂量组脑组织总蛋白含量(0.76±0.25)g/L比(0.55±0.12)g/L]增加(P<0.05).与乙醇模型组比较,肉苁蓉多糖小、中、大剂量组逃避潜伏期(22.67±18.35)s、(22.15±16.22)s、(18.00±13.44)s比(51.33±22.19)s]、第1次到达站台时间(16.70±11.25)s、(19.75±14.62)s、(9.47±5.46)s比(30.09±13.63)s]缩短(P<0.05),肉苁蓉多糖小、大剂量组穿越站台次数(5.15±1.28)次、(4.83±0.75)次比(1.34±0.83)次]增加(P<0.05),肉苁蓉多糖大剂量组脑组织总蛋白(0.76±0.25)g/L比(0.56±0.12)g/L]含量增加(P<0.05).结论 肉苁蓉多糖可明显改善小鼠再现性记忆障碍、巩固性记忆障碍,其机制与促进脑内蛋白质合成有关.

Effect of mongolian medicine cistanche polysaccharides on learning and memory in mice

Objective To observe the effect of cistanche deserticola polysaccharides on learning and memory ability of different types of spatial learning and memory abilities in micey.Methods The 120 mice were randomly divided into blank group, model group, Cistanche deserticola polysaccharide, low and medium dose group, and Lacita group, 20 rats in each group. The low, medium and large dosage groups were given 25, 50 and 100 mg/kg Cistanche deserticola polysaccharides, piracetam group was given 10 mg/kg Laci Staw, blank group and model group were given 10 mg/kg volume distilled water. Continuous administration for 6 weeks. Cicloheximide mice were used to consolidate memory impairment model, using ethanol to establish a model with reappearance of memory disorders. The ability of learning and memory in water maze test in mice, the mice were detected in the brain of total protein, MDA, SOD.Results Compared with model group, the escape latency (9.45 ± 2.86 s, 12.73 ± 10.89 svs. 48.15 ± 30.33 s), and the first time arrived at the station (19.33 ± 3.27 s, 13.81 ± 9.79 svs. 40.71 ± 16.76 s) was the median, large dose group were significantly shortened (P<0.05); the first time to reach the site (11.58 ± 7.04 svs. 40.71 ± 16.76 s) in the low dose group was significantly shortened (P<0.05), the number of crossing the platform (5.46 ± 2.09vs. 3.03 ± 1.47) in the low dose group significantly increased (P<0.05); the total protein content in brain tissue (0.76 ± 0.25 g/Lvs. 0.55 ± 0.12 g/L) in the high dose group significantly increased (P<0.05). Compared with the ethanol model group, the escape latency (22.67 ± 18.35 s, 22.15 ± 16.22 s, 18.00 ± 13.44 svs. 51.33 ± 22.19 s), the first time arrived at the station (16.70 ± 11.25 s, 19.75 ± 14.62 s, 9.47 ± 5.46 svs. 30.09 ± 13.63 s) in the low, medium and high dose group were significantly shortened (P<0.05), crossing the target (5.15 ± 1.28, 4.83 ± 0.75vs. 1.34 ± 0.83) in the low, medium and high dose group were significantly increased (P<0.05).Conclusions Cistanche polysaccharide could significantly improve the reproducibility of memory impairment in mice.