丝光绿蝇抗菌物质的超声波诱导

目的探讨超声波对丝光绿蝇抗菌物质的诱导表达规律。方法丝光绿蝇初三龄幼虫经0 W、40 W、80 W、120 W功率超声波处理、经100 W超声波处理3 min、5 min、7 min和超声波诱导后不同时间提取血淋巴,观察其对溶壁微球菌的抑菌效果。结果0 W、40 W、80 W、120 W超声波处理5 min,诱导后48 h提取的血淋巴对溶壁微球菌的抑菌环直径(mm)分别为:14.33±0.58、21.33±1.04、20.83±0.58、16.33±0.58。100 W分别处理3 min、5 min、7 min,诱导后48 h的抑菌环直径(mm)分别为:18.67±1.26、20.33±0.76、20.17±0.29。100 W处理5 min,诱导后0 h、24 h、48 h、72 h的抑菌环直径(mm)分别为:14.33±0.58、19.83±0.29、20.33±0.76、20.00±0.50。结论超声波可诱导丝光绿蝇抗菌物质表达,以超声波100 W、5 min诱导后48 h提取为宜。

Antibacterial substances of Lucilia sericata induced by ultrasonic treatment

Objective To investigate the expressive regularity of antibacterial substances of Lucilia sericata induced by ultrasonic treatment. Methods Three instar larvas of Lucilia sericata were treated by 0 W, 40 W, 80 W, 120 W ultrasonic respectively, and were treared for 3 min,5 min,7 min and for different hours by 100 W ultrasonic. Then collecting the hemolymph to observe the bacteriostatic effect angist Micrococcus lysodeikticus. Results Hemolymph was treated by 0 W,40 W,80 W,120 W ultrasonic for 5 min, after being induced for 48 h, the diameters （ram） of bacteriostatic rings were respectively recorded as fol- lows： 14.33 ± 0.58,21.33 ± 1.04,20.83±0.58,16.33 ± 0.58. Hemolymph was treated by 100 W ultrasonic for 3 rain,5 min,7 min after being induced for 48 h,the diameters（mm） of bacteriostatic rings were： 18.67 ± 1.26,20.33 ± 0.76,20.17 ± 0.29. Hernolymph was treated by 100 W ultrasonic for 5 min,after being induced for 0 h,24 h,48 h and 72 h,the diameters（mm） of bacteriostatic rings were ： 14.33 ±0.58,19.83 ± 0.29,20.33 ± 0.76,20.00 ± 0.50. Conclusion Expression of antibacterial substances of Lucilia sericata can be induced by ultrasonic treatment and it is best to collect hemolymph after being induced for 48 h by 100 W ultrasonic for 5min.