从剖宫者产羊水和宫颈标本中检出沙眼衣原体的研究

目的；三文通过检查配对母胎标本中的沙眼衣原体（Ｃｒ），拟探讨Ｃｔ垂直传播的机制。方法：应用细胞培养，聚合酶链反应（ＰＣＲ）检查配对对母胎标本（宫颈、羊水、新生儿睑结膜）中的沙眼衣原体（Ｃｒ）。结果：１０２例剖宫产配对母胎标本中，宫颈、羊水、新生儿睑结膜均为阳性有２４例。宫颈阴性、羊水、新生儿睑结膜阳性共６例。宫颈阳性、羊水、新生儿睑结膜阴性共４例。宫颈、羊水、新生儿睑结膜均为阴笥有６８例。孕妇的Ｃ

Detection of chlamydia trachomatis from amniotic fluid and cervical samples at caesarean, birth

Objective: To study the modes and routes of the vertical Chlamydia trachomatis(Ct) transmission. Methods:Chlamydia trachomatis in the matched maternal-fetal samples(cervices, amniotic fluid and neonatal conjunctivas) was detected by cell culture and polymerase chain reaction(PCR). Results:Of the 102 matched maternal-fetal samples at caesarean birth ,24 matched samples of cervices, amniotic fluid and neonatal conjunctivas were positive for Ct by cell culture and PCR; 6 were negative for cervices but positive for amniotic fluid and neonatal conjunctivas; 4 were positive for cervices but negative for amniotic fluid and neonatal conjunctivas; 68 were negative for cervices, amniotic fluid ,and neonatal conjunctivas. The Ct transmission rates of pregnant women and newborns were 27.5%(28/102) and 29.5%(30/102), respectively. Conclusion:Cervical Chlamydia infection was highly related to the amniotic infection.The Ct infection in pregnant women may cause fetal infection and the pathways of intrauterine infection seem to be ascension from cervical access to the amniotic cavity.