Amino acid sequence similarity of Hev b 3 to two previously reported 27- and 23-kDa latex proteins allergenic to spina bifida patients |
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Authors: | H.Y. Yeang M.A. Ward A.S.M. Zamri M.S. Dennis D.R. Light |
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Affiliation: | Biotechnology and Strategic Research Division, Rubber Research Institute of Malaysia, Kuala Lumpur, Malaysia;Glaxo-Wellcome Research &Development, Stevenage, UK;Genentech, Inc. San Francisco, CA, USA |
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Abstract: | Separate studies have reported spina bifida patients to be especially allergic t o proteins of 27 and 23 kDa found in the serum of centrifuged natural rubber latex. An insoluble latex protein located on the surface of small rubber particles, Hev b 3, has similarly been found to be allergenic to spina bifida patients. In this study, internal amino acid sequences of Hev b 3 showed similarity to the published sequences for the 27- and 23-kDa latex proteins. The latter allergens are hence identified as Hev b 3. Determination of the molecular weight of Hev b 3 revealed various species of 22–23 kDa. The consistent gaps of about 266 Da observed between various forms of the intact protein suggest that the protein undergoes post-translational modification. To determine whether Hev b 3 also occurs i n a soluble form in the latex serum, its presence in molecular-filtered serum was checked by ELISA and Western blot, TTie results showed Hev b 3 to be largely absent in the C-serum from fresh latex. The protein is therefore insoluble in its native state. However, a small amount of the solubilized protein was detected in ammonia-stabilized latex (commonly used in the manufacture of latex products). |
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Keywords: | amino acid sequence Hev b 3 Hevea brasiliensis latex allergy latex protein mass spectrometry rubber particle spina bitida |
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