| 高效液相色谱法测定复方氢化可的松栓中醋酸氢化可的松的含量 |
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| 引用本文： | 高同舟,慕芳英,韩映晨,张晓玲,郑美善,谭荭.高效液相色谱法测定复方氢化可的松栓中醋酸氢化可的松的含量[J].延边医学院学报,2002,25(2):105-108. |
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| 作者姓名： | 高同舟 慕芳英 韩映晨 张晓玲 郑美善 谭荭 |
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| 作者单位： | [1]延边药品检验所，吉林延吉133000 [2]敖东药业延吉公司，吉林延吉133000 |
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| 摘 要： | 目的 ]测定复方氢化可的松栓中醋酸氢化可的松的含量 .方法 ]用高效液相色谱法 ,采用KromasilC18柱 ,以甲醇 水 36 0mL/L醋酸 (6 0∶4 0∶1)为流动相 ,流速为 0 8mL/min ,检测波长为 2 4 2nm .结果 ]醋酸氢化可的松在 0 0 3～ 0 15 μg范围内呈良好的线性关系 (r =0 9999) ,回收率为99 2 1% ,相对标准偏差为 0 2 4 % .结论 ]高效液相色谱法在测定复方氢化可的松栓中醋酸氢化可的松的含量时灵敏、快速、准确
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| 关 键 词： | 氢化可的松 栓剂 色谱法 高压液相 |
| 文章编号： | 1000-1824(2002)02-0105-03 |
| 修稿时间： | 2002年4月26日 |
Determination of hydrocortsone acetate in compound hydrocortisone suppository by high performance liquid chromatography |
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| GAO Tong-zhou,MU Fang-ying,HAN Ying-chen,ZHANG Xiao-ling,ZHENG Mei-shan,TAN Hong.Determination of hydrocortsone acetate in compound hydrocortisone suppository by high performance liquid chromatography[J].Journal of Medical Science Yanbian University,2002,25(2):105-108. |
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| Authors: | GAO Tong-zhou MU Fang-ying HAN Ying-chen ZHANG Xiao-ling ZHENG Mei-shan TAN Hong |
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| Abstract: | OBJECTIVE?To detect hydrocortisone acetate in compound hydrocortisone suppository.?METHODS?Using high performance liquid chromatography,the Kromasil C 18 column, mobile phaseconsisting of MeOH∶H 20∶360?mL/L glacial acid(60∶40 ∶1 ).The detection wavelength was at 242?nm of detecting wavelength and 0 8?mL/min of flow rate.?RESULTS?The liner range of hydrocortisone was 0 03～0 15?μg( r =0 999?9), and the recovery was 99 21% with a RSD of 0 24%.?CONCLUSION?The method of HPLC is sensitive, rapid and accurate for detecting the hydrocortisone. |
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| Keywords: | hydrocortisone suppositories chromatography high pressure liquid |
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