| 结核分支杆菌镜检、培养和PCR技术诊断结核病的临床评估 |
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| 引用本文： | 肖爱清,侯双翼,李国明,钱辉,皇甫永穆. 结核分支杆菌镜检、培养和PCR技术诊断结核病的临床评估[J]. 中国防痨杂志, 2003, 25(2): 68-70 |
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| 作者姓名： | 肖爱清 侯双翼 李国明 钱辉 皇甫永穆 |
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| 作者单位： | 1.湖北省医学科学院结研所 武汉 430079；2同济医科大学医学分子生物学研究室 武汉 430030； |
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| 摘 要： | 目的 评估结核分支杆菌PCR、培养、镜检对结核病诊断的临床效果。方法 对1217例临床可疑结核病人用PCR、培养和镜检三种方法的阳性检出率进行比较。结果 不同临床标本用PCR技术诊断结核病,其敏感性高于培养和涂片镜检,阳性检出率分别为:88.7%,43.4%,26.5%。结论 通过三种方法比较分析,说明PCR技术是一种速度快、检出率高的检测结核分支杆菌的重要方法,对结核病的诊断有重要的参考价值。
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| 关 键 词： | 结核 肺/诊断 聚合酶链反应 培养 镜检 |
| 修稿时间： | 2001-04-04 |
Clinical evaluate the diagnostic value of tuberculosis for detection of M.tuberculosis PCR,culture and microscopy assay |
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| XIAO Ai qing,HOU Shuang yi,LI Guo ming,et al.. Clinical evaluate the diagnostic value of tuberculosis for detection of M.tuberculosis PCR,culture and microscopy assay[J]. The Journal of The Chinese Antituberculosis Association, 2003, 25(2): 68-70 |
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| Authors: | XIAO Ai qing HOU Shuang yi LI Guo ming et al. |
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| Affiliation: | Department of Tuberculosis Control in Hubei Medical Scientific Academy,Wuhan 430079 |
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| Abstract: | Objective To study the clinical effect of M.tuberculosis examination by PCR,culture and microscopy assay.Methods 1?217 specimens of the clinical suspicious tuberculosis cases were compared with 3 kinds of Methods (PCR,culture and microscopy) in diagnosis of tuberculosis.Results The positive rate of PCR?culture and smear by the different clinical specimens were 88.7%,43.4%,26.5% respectively.Conclusion By means of analyze of comparation with 3 kinds of methods,PCR is the fast and its positive examiniatorial rate is the highest.For diagnosis of tuberculosis,it is one of important Methods in detection of M.tuberculosis. |
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| Keywords: | Tuberculosis pulmonary/diagnose PCR Culture Microscopy |
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