母儿间单纯疱疹病毒感染的研究

目的 研究母儿间单纯疱疹病毒(HSV)感染及其垂直传播状况。方法 应用酶联免疫吸附试验(ELISA)筛查455例孕妇HSV特异性抗体(IgM、2型和1型IgG)，并用多聚酶链反应(PCR)检测抗体阳性孕妇的胎儿组织及其附属物中HSV2型DNA的表达。结果 孕期HSV1型和2型感染率分别为85．27％、47．69％。异常组HSV2型IgG抗体的检出率明显高于对照组。HSV宫内感染率为4．24％。羊水、胎盘及胎儿组织中均可检出HSV2型DNA。结论 妊娠期HSV感染较常见，其中HSV2型感染与流(早)产、畸形等异常孕产史关系密切。ELISA法结合PCR技术是诊断HSV宫内感染的一种行之有效的方法。

A Study on HSV Infection between Pregnant Women and Their Fetus/Newborns

Objective To study the state of herpes simplex virus (HSV) infection and the vertical transmission between pregnant women and their fetus/newborns. Methods ELISA was applied to screen HSV specific antibodies in 455 pregnant women. HSV type 2 DNA was detected by PCR technique in the tissues and appendages of fetus, whose mothers had positive HSV IgM and/or type 2 IgG antibodies. Results The infective rate of HSV types1 and 2 during pregnancy were 85.27% and 47.69%, respectively. The intrauterine infective rate was 4.24%. The positive rate of HSV type 2 IgG in the pregnant women with history of abnormal pregnancy was significantly higher than that in the control group. HSV type 2 DNA was found in the amniotic fluid and tissues of placenta and fetus. Conclusion The HSV infection was common during pregnancy. HSV type 2 infection was closely related to the abnormal pregnancy, such as abort, premature delivery and malformation. ELISA combined with PCR technique is a valuable method for the diagnosis of HSV infection.