| 表皮生长因子受体的敲除对小鼠骨密度以及成骨细胞募集的影响 |
| |
| 引用本文： | 王珂,李然伟,刘永红,赵燕颖,杨世杰.表皮生长因子受体的敲除对小鼠骨密度以及成骨细胞募集的影响[J].吉林大学学报(医学版),2008,34(3):425-428. |
| |
| 作者姓名： | 王珂 李然伟 刘永红 赵燕颖 杨世杰 |
| |
| 作者单位： | 吉林大学第二医院呼吸内科,吉林长春,130041;吉林大学第二医院泌尿外科,吉林长春,130041;吉林省肿瘤医院,吉林长春,130012;吉林大学基础医学院生理学教研室,吉林长春,130021;吉林大学基础医学院药理学教研室,吉林长春,130021 |
| |
| 摘 要： | 目的：探讨表皮生长因子受体（EGFR）信号转导系统在小鼠骨胳发育中的作用。 方法：以EGFR敲除鼠(EGFR-/-)为实验动物模型，通过马森三色染色（Masson Trichrome）、原位杂交技术，观察其子1代胚胎鼠及新生鼠四肢长骨发育过程中松质骨的形成及成骨细胞募集的变化。 结果:形态学观察分析， EGFR-/-松质骨的形成较正常野生鼠(EGFR+/+)延迟，骨小梁较正常鼠减少；在胚胎发育第16.5天 (E16.5), 其成骨细胞向中心生长板的募集速度较EGFR+/+亦延迟； 形态学观察EGFR-/-和EGFR+/+核心结合因子A （Cbfa）、降钙素 （OC）及I 型胶原（Col I）在表达数量上有明显不同。结论:EGFR信号转导系统在在小鼠松质骨的形成过程中起着重要作用，其机制可能与调节成骨细胞向中心生长板的募集有关。
|
| 关 键 词： | 受体 表皮生长因子 骨发育 松质骨 成骨细胞 |
| 收稿时间： | 2007-09-05 |
Effect of epidermal growth factor receptor deficiency on trabecular bone formation and recruitment of osteoblasts in mice |
| |
| WANG Ke,LI Ran-wei,LIU Yong-hong,ZHAO Yan-ying,YANG Shi-jie.Effect of epidermal growth factor receptor deficiency on trabecular bone formation and recruitment of osteoblasts in mice[J].Journal of Jilin University: Med Ed,2008,34(3):425-428. |
| |
| Authors: | WANG Ke LI Ran-wei LIU Yong-hong ZHAO Yan-ying YANG Shi-jie |
| |
| Institution: | 1. Department of Respiratory Medicine, Second Hospital, Jilin University, Changchun 130041, China; 2.Department of Urinary Surgery, Second Hospital, Jilin University, Changchun 130041, China: 3.Tumor Hospital of Jilin Province, Changchun 130012,China; 4.Department of Physiology,School of basic Medical Sciences, Jilin University,Changchun 130021,China;5.Department of Pharmacology, School of basic Medical Sciences , Jilin University, Changchun 130021, China |
| |
| Abstract: | Objective To determine the role of epidermal growth factor receptor(EGFR) signaling in bone development in mice.Methods Long bone from EGFR wild type(EGFR+/+) and knockout mice(EGFR-/-) were collected and analyzed by Trichrome Masson staining,in situ hybridization was used to observe trabecular bone formation and recruitment of osteoblasts.Results EGFR deficiency can cause delayed trabecular bone formation in EGFR-/-mice and delayed osteoblast recruitment compared with EGFR+/+ mice.There were different expressions of core binding factor A(Cbfa), osteocalcin(OC) and collagen I(Col I) between EGFR+/+ and EGFR-/-.Conclusion EGFR signaling play an important role in trabecular bone formation,the partial mechanisms for which might be related to the regulation of osteoblast recruitment. |
| |
| Keywords: | receptor epidermal growth factor bone development brabecular bone osteoblasts |
| 本文献已被 维普 万方数据 等数据库收录！ |
| 点击此处可从《吉林大学学报(医学版)》浏览原始摘要信息 |
| 点击此处可从《吉林大学学报(医学版)》下载免费的PDF全文 |
|
