用浓缩地鼠肾细胞狂犬病毒抗原免疫马匹获得高效价血浆

目的制备高效价马抗狂犬病免疫血浆。方法通过细胞制备、病毒的接种、培养、收获、浓缩等步骤，研制了浓缩地鼠。肾细胞狂犬病毒抗原，并与羊脑狂犬病毒抗原作马匹免疫对比试验。结果浓缩地鼠。肾细胞抗狂犬病免疫血浆的效价明显高于羊脑抗狂犬病免疫血浆。试验组血浆平均效价为157IU／ml，对照组为113IU／ml，两组差异有统计学意义。结论已成功制备高效价抗狂犬病免疫血浆。

High Titer Anti-rabies Horse Plasma Preparation with Rabies Virus Propagated in Primary Hamster Kidney Cell Cultures

Objective To prepare high-titer horse anti-rabies plasma.Methods Virus inoculation,cultivation and harvest were adopted to generate concentrated rabies virus antigen cultured in primary hamster kidney cells.Immunological effects of primary hamster kidney cell-derived and sheep brain-derived rabies virus on horse were compared.Results The plasma titers of anti-rabies antibodies were significantly higher in horses immunized with primary hamster kidney cell-derived rabies virus(mean,157 IU/ml)than in those immunized with sheep brain-derived rabies virus(mean,113 IU/ml).Conclusion High titer horse anti-rabies plasma was successfully prepared.