西洋参胶囊对手机频率电磁辐射大鼠肝脏组织氧化损伤及Nrf2蛋白表达的影响

目的观察西洋参胶囊对900 MHz手机频率电磁辐射大鼠肝脏组织氧化损伤和肝Nrf2蛋白表达的影响。方法40只雄性SD大鼠随机分为正常组、模型组、水飞蓟宾组和西洋参组，每组10只。正常组不接受辐射，模型组、水飞蓟宾和西洋参组每天暴露于900 MHz手机频率辐射4 h，连续12天。水飞蓟宾组和西洋参组辐射同时分别灌服水飞蓟宾胶囊混悬液和西洋参胶囊混悬液（1 mL/200 g体重），正常组和模型组每日灌服生理盐水。采用HE染色观察肝脏组织形态学变化，比色法检测大鼠肝组织MDA、SOD、GSH、GSH-PX含量，免疫组化法、Western blot 检测大鼠肝Nrf2蛋白表达。结果与正常组比较，模型组大鼠肝细胞核缩小或部分消失，肝组织MDA含量及Nrf2蛋白表达升高（P〈0.05，P〈0.01），SOD、GSH含量降低（P〈0.05）。与模型组比较，水飞蓟宾和西洋参组大鼠肝细胞核固缩均明显减轻，接近正常；水飞蓟宾组MDA含量及Nrf2蛋白表达降低（P〈0.05），SOD、GSH、GSH-PX含量升高（P〈0.05）；西洋参组MDA含量及Nrf2蛋白表达降低（P〈0.05），SOD、GSH含量显著性增加（P〈0.01，P〈0.05）。结论900 MHz手机频率电磁辐射可影响大鼠肝细胞Nrf2表达，致氧化损伤，引起肝细胞形态异常；水飞蓟宾和西洋参可促进肝细胞形态的修复，其机制可能与影响大鼠肝细胞Nrf2表达，减轻氧化损伤有关。

Effect of American Ginseng Capsule on the Liver Oxidative Injury and the Nrf2 Protein Expression in Rats Exposed by Electromagnetic Radiation of Frequency of Cell Phone

Objective To observe the effect of American Ginseng Capsule （AGC） on the liver oxidative injury and the Nrf2 protein expression in the liver tissue of rats exposed by 900 MHz cell phone electromagnetic radiation. Methods Totally 40 male SD rats were randomly divided into the normal con- trol group, the model group, the Shuifei Jibin Capsule （SJC） group, and the AGC group,10 in each group. Rats in the normal control group were not irradiated. Rats in the rest three groups were exposed by imitated 900 MHz cellular phone for 4 h in 12 consecutive days. Meanwhile, rats in the SJC group and the AGC group were intragastrically administrated with suspension of SJC and AGC （1 mL/200 g body weight） respectively. Normal saline was administered to rats in the normal control group and the model group. The histolomorphological changes of the liver tissue were observed by HE staining. Contents of m alonic dialdehyde （MDA）, superoxide dismutase （SOD）, glutathione （GSH）, and glutathione peroxidase （GSH-PX）were detected by colorimetry. The Nrf2 protein expression of hepatocytes was detected by immunohistochemical assay and Western blot. Results Compared with the normal control group,hepatocyte nucleus was atrophied or partially disappeared, the contents of liver MDA and Nrf2 protein obviously increased （P 〈0.05, P 〈0.01 ） ; contents of liver SOD and GSH decreased （P 〈0.05） in the model group. Compared with the model group, karyopyknosis was obviously attenuated and approached to the normal level in the SJC group and the AGC group. The contents of liver MDA and Nrf2 protein expression decreased （P 〈0.05）, and the contents of liver SOD, GSH, and GSH-PX obviously increased （P 〈 0.05） in the SJC group. The contents of liver MDA and the Nrf2 protein expression decreased （P 〈 0.05）, and contents of SOD and GSH obviously increased in the AGC group （P 〈0.01, P 〈0.05）. Conclusions The electromagnetic radiation induced by 900 MHz cell phone could affect the expression of Nrf2 protein, induce oxidative injury, and induce abnormal morphology of liver cells. SJC and AGC could promote the morphological recovery of the liver cells. Its mechanism might be related to affecting the expression of Nrf2 protein and attenuating oxidative damage of liver cells.