止嗽散加减方对慢性阻塞性肺疾病西北寒燥证大鼠模型肺功能的影响

目的观察止嗽散加减方对慢性阻塞性肺疾病（chronic obstructive pulmonary diseases，COPD）西北寒燥证大鼠模型肺功能的影响。方法90只雄性Wistar大鼠随机分为空白组（20只）、COPD模型组（模型组，35只）和COPD西北寒燥证组（寒燥组，35只）。利用气管滴注弹性蛋白酶结合熏烟90天的方法建立COPD模型，气管滴注弹性蛋白酶、熏烟结合寒燥环境应激90天的方法建立COPD西北寒燥证模型；后将寒燥组随机分为止嗽散加减方干预组（中药组，11只）和生理盐水组（对照组，10只）各组连续干预15天。在第90天和第105天时分别用小动物肺功能仪检测各组大鼠的肺功能，并观察其肺部病理。结果模型组和寒燥组气道内均可闻及少量痰鸣音，病理切片示肺泡扩张，间隔变窄，断裂并融合成较大囊腔，有炎症细胞浸润，寒燥组出现畏寒、饮水量增多和便质湿烂不成形表现，止嗽散加减方干预后症状改善。肺功能检测，与空白组比较，模型组Te升高（P〈0.01），EF50降低（P〈0.05）；寒燥组PEF及EF50降低（P〈0.01），Ti及Te升高（P〈0.01，P〈0.05）。与对照组比较，中药组干预后PEF及EF50升高（P〈0.01），Ti及Te降低（P〈0.01）。结论止嗽散加减方可以改善COPD西北寒燥证大鼠模型的症状表现，延缓其肺功能下降的速度。

Effect of Modified Zhisou Powder on the Lung Function of Chronic Obstructive Pulmonary Disease Model Rats of Northwest China Cold Dryness Syndrome

Objective To observe Modified Zhisou Powder （MZP） on the lung function of chronic obstructive pulmonary disease （COPD） model rats of northwest China cold dryness syndrome （NCCDS）. Methods Totally 90 male Wistar rats were randomly divided into three groups, i.e., the normal control group （n =20）, the COPD model group （n =35）, and the COPD of NCCDS group （n =35）. The COPD model was established by tracheal dripping porcine pancreatic elastase （PEE） in combination with fumigation for 90 days. The COPD of NCCDS model was set up by tracheal dripping PEE ＋ fumigation ＋ cold and dry environmental stress for 90 days. Then rats in the COPD of NCCDS were randomly divided into the MZP intervention group （n =11 ） and the normal saline intervention group （n =10）. All intervention lasted for 15 successive days. The lung function was detected using Small Animal Lung Function Device at day 90 and day 105. And the lung pathology was also observed. Results Little amount of sputum sound could be heard in the airway of the COPD model group and the COPD of NCCDS group. Pathological section showed alveolar ectasia, narrowed and broken alveolar septa, forming larger capsular space with infiltration of inflammatory cells. Rats in the COPD of NCCDS group showed chills, increased amount of drinking water, and loose stool. MZP could improve their symptoms. As for lung function test, compared with the normal control group, Te increased in the COPD model group （P 〈0.01 ）, and EF50 decreased （P 〈0.05）. PEF and EF50 decreased （P 〈0.01）, Ti and Te increased （P 〈0.01, P 〈0.05） in the COPD of NCCDS group. Compared with the normal saline intervention group, PEF and EF50 increased （P 〈 0.01 ）, Ti and Te decreased （P 〈0.01 ） in the MZP intervention group. Conclusion MZP could improve the symptoms of COPD rats of NCCDS, and delay the velocity of decreased lung function.