消痰散结方对MKN-45人胃癌裸鼠原位移植瘤微卫星不稳定的抑制作用观察

目的观察消痰散结方对MKN-45人胃癌裸鼠原位移植瘤微卫星不稳定的抑制作用。方法采用MKN-45人胃癌细胞株建立裸鼠皮下瘤模型皮下传3代作为实验模型的瘤源，采用OB胶粘合法建立人胃癌裸鼠原位移植瘤模型。30只模型裸鼠分为模型组、中药组和化疗组，每组10只。中药组给予消痰散结方（0.4 mL/天）灌胃，化疗组给予喃氟啶0.4 mL/天灌胃，模型组无干预措施。用药6周后，测瘤质量、计算抑瘤率，检测5个微卫星不稳定位点（D17S250、D2S123、D5 S346、Bat-25和BAT26）的大小、峰高度及峰面积。结果中药组抑瘤率为40.84%，中药组的瘤质量明显低于模型组（P〈0.01），与化疗组比较，差异无统计学意义（P〉0.05）。模型组高度微卫显不稳定发生率为70%，低度微卫星不稳定发生率为30%，使用消痰散结方治疗6周后，微卫星位点趋于稳定，均表现为微卫星稳定。结论消痰散结方对微卫星不稳定MKN-45人胃癌裸鼠原位移植瘤有抑制作用。

Inhibitory Effect of Xiaotan SanJie Recipe on the Microsatellite Instability of Orthotopic Transplantation Tumor in MKN-45 Human Gastric Cancer Nude Mice

Objective To study the inhibitory effect of Xiaotan Sanjie Recipe （XSR） on the microsatellite instability of orthotopic transplantation tumor in MKN-45 human gastric cancer nude mice. Methods The 3rd passage subcutaneous transplantation tumor was taken as the origin of the model by using MKN-45 human gastric cancer cell lines. MKN-45 human gastric cancer nude mouse model was established using OB glue adhesive method. Then 30 nude mice were divided into the model group, the XSR group, and the chemotherapy group. Mice in the XSR group were intragastricalty given XSR at the daily dose of 0.4 mL. Mice in the chemotherapy group were intragastrically given Fluorouracil at the daily dose of 0.4 mL. No intervention was given to mice in the model group. After 6 weeks of medication, the tumor weight was measured, and the tumor inhibition rate calculated. The size, the peak height, and the peak area of 5 microsatellite instability sites were detected. Results The tumor inhibition rate was 40.84% in the XSR group. The tumor weight was significantly lower in the XSR group than in the model group （P 〈 0.01 ）, showing no statistical difference when compared with the chemotherapy group （P 〉0.05）. The incidence of high microsateUite instability （MSI-H） in the model group was 70%, and the incidence of low microsatellite instability （MSI-L） was 30%. Microsatellite stable site tended be stable after 6 weeks of XSR treatment. Conclusion XSR showed inhibition on microsatellite instable orthotopic transplantation tumor in MKN-45 human gastric cancer nude mice.