| 补骨脂与蛇床子抑制乳腺癌骨转移的体内实验 |
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| 引用本文： | 李琼,刘胜,杨顺芳. 补骨脂与蛇床子抑制乳腺癌骨转移的体内实验[J]. 上海中医药大学学报, 2010, 0(1): 53-58 |
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| 作者姓名： | 李琼 刘胜 杨顺芳 |
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| 作者单位： | 上海中医药大学附属龙华医院乳腺科;复旦大学附属妇产科医院乳腺科;上海交通大学附属胸科医院核医学科; |
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| 基金项目： | 国家自然科学基金资助项目(30772811); 上海市重点学科建设项目(T0304); 上海市高等学校科学技术发展基金项目(05CZ13); 上海市教委曙光跟踪项目(08GG12) |
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| 摘 要： | 目的:探讨温肾药补骨脂、蛇床子及其主要成分对乳腺癌骨转移的抑制作用。方法:将雌性BALB/c裸小鼠随机分为生理盐水组、表柔比星组、补骨脂素组、蛇床子素组和中药煎剂(蛇床子+补骨脂)组。采用人乳腺癌骨高转移细胞株MDA-MB-231BO注射裸鼠左心室制作乳腺癌骨转移动物模型,予药物干预后评价各组骨转移情况,并通过免疫组化法测定各组裸鼠骨转移灶中甲状旁腺激素相关蛋白(PTHrP)、护骨素(OPG)、核因子kB受体活化因子配基(RANKL)的蛋白表达量。结果:与生理盐水组比较,各药物组均能明显抑制裸鼠乳腺癌骨转移(P0.05),其中补骨脂素、蛇床子素的效果优于中药煎剂,且蛇床子素的抑制作用最强;各药物组均能明显降低裸鼠骨转移灶中PTHrP和RANKL蛋白表达(P0.05),其中补骨脂素、蛇床子素可以明显升高裸鼠乳腺癌骨转移灶中OPG蛋白表达(P0.05)。结论:补骨脂、蛇床子及其主要成分可以在体内实验中抑制乳腺癌骨转移,其中中药单体的效果优于中药粗制剂。其作用机制可能与抑制乳腺癌细胞生长,降低乳腺癌细胞分泌促骨吸收因子PTHrP的量,从而降低成骨/基质细胞RANKL的表达,同时促进OPG的表达有关。
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| 关 键 词： | 乳腺癌骨转移 温肾药 补骨脂 蛇床子 抗转移 |
Inhibition of Bone Metastasis of Breast Cancer by Fructus Psoraleae and Fructus Cnidii in vivo |
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| LI Qiong LIU Sheng YANG Shun-fang. Inhibition of Bone Metastasis of Breast Cancer by Fructus Psoraleae and Fructus Cnidii in vivo[J]. Acta Universitatis Traditionis Medicalis Sinensis Pharmacologiaeque Shanghai, 2010, 0(1): 53-58 |
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| Authors: | LI Qiong LIU Sheng YANG Shun-fang |
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| Affiliation: | LI Qiong~(1,2) LIU Sheng~1 YANG Shun-fang~3 1.Department of Galactophore Surgery,Longhua Hospital Affiliated to Shanghai University of TCM 2.Department of Galactophore Surgery,Obstetrics & Gynaecology Hospital Affiliated to Fudan University 3.Department of Nuclear Medicine,Shanghai Chest Hospital Affiliated to Shanghai Jiaotong University |
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| Abstract: | Objective:To study the inhibiting effect of Fructus Psoraleae and Fructus Cnidii as well as their major ingredients on the bone metastasis of breast cancer.Methods:The female BALB/c nude mice were randomly divided into saline solution(NS) group,epirubicin group,psoralen group,osthol group and Chinese herbal medicine Fructus Psoraleae+Fructus Cnidii(FPFC) group.The animal model was established by injecting human breast cancer cells MDA-MB-231 BO into the left cardiac ventricle of nude mice.The mice in differ... |
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| Keywords: | Bone metastasis of breast cancer kidney warming herbs Fructus Psoraleae Fructus Cnidii anti-metastasis |
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