盖诺与顺铂联合化疗加同期放射对乳腺癌MCF-7细胞作用的研究

目的：探讨盖诺（NVB）联合顺铂（DDP）的放化同期治疗乳腺癌的有效性、时机选择和作用机制。方法：NVB和DDP（NP方案）对乳腺癌MCF-7细胞联合化疗同期放射。比较照射加联合化疗组和单纯照射组、不同间隔时间联合化放疗的细胞存活率（SF）、凋亡和细胞周期阻滞差异。结果：联合化疗加同期放射后SF较单独放疗更低。化疗处理后间隔4、12和36h照射的SF值最低，0h居中，而阃隔48和72h后，SF值明显上升，放化疗协同杀灭效应减弱，SF最低与最高值相差约15倍。联合化疗加照射组细胞凋亡指数（AI）明显高于单独放射和联舍化疗组。6、8Gy放射后36h时，放化疗联合组与单独放射组比较A1分别高达30．7％、38．35％和2．22％、3．27％，差异有统计学意义，P〈0．05。化疗处理MCF-7细胞1h后在间隔4、12、36和72h处，G2／M周期细胞比例较高，0.48h处较低。结论：联合化放疗对乳腺癌MCF-7细胞具有较强的协同杀灭作用，化放联合治疗的时机选择在肿瘤细胞杀灭过程中具有重要的意义，这种协同杀灭作用与增加细胞凋亡率和化疗造成的细胞G2／M周期阻滞有关。

Study of NVB and DDP chemotherapy with concurrent radiotherapy on breast cancer MCF-7 cells

OBJECTIVE: To explore the effectivity of concurrent radiotherapy and chemotherapy, opportunity chosing and mechanism on breast cancer MCF-7 cells. METHODS: The results of survival fractions (SF) among the groups of combination of NVB and DDP with concurrent radiotherapy, chemotherapy and radiotherapy alone were compared. Further researches on apoptosis, cell cycle accumulation within these groups were done. RESULTS: SF of the group combined chemotherapy with radiation was much lower than that of group of radiotherapy alone. The SFs of MCF-7 cells irradiated at 4 h, 12 h and 36 h after the chemotherapy were the lowest. SF at 0 h was middling. SFs at 48 h and 72 h after the chemotherapy were found rising greatly. The highest SF was about 15 times of the lowest. Apoptosis index (AI) of the combination group of chemotherapy and radiation was much higher than those of groups of chemotherapy and radiation alone. Especially at 36 h after 6 Gy or 8 Gy by being irradiated, AIs of the combination group of chemotherapy and radiation were 30.7% and 38.35% while AIs of group of radiation alone were 2.22% and 3.27%, P<0.05. G2/Ms of MCF-7 cell at 4, 12, 36 and 72 hours after chemotherapy were higher compared with those at 0 and 48 hours later. CONCLUSIONS: Teh combination of concurrent chemotherapy with radiotherapy can make much stronger cooperated killing action on MCF-7 cells. Opportunity of the combination treatment takes a great significance during this process. This kind of cooperated killing is related to the increase of apoptosis from combination of chemotherapy with radiation and cell cycle accumulation at G2/M caused by chemotherapy.