载银珊瑚羟基磷灰石的生物相容性评价

目的 评价负载8×10-5 mol/L银离子后珊瑚羟基磷灰石(CHA)的生物相容性.方法 采用珊瑚羟基磷灰石材料与AgNO3及NaCl复合,经真空冷冻干燥后制备成8×10-5 mol/L Ag+浓度的载银珊瑚羟基磷灰石(Ag-CHA),并对健康成年昆明小白鼠、新西兰大白兔、白化豚鼠进行了全身急性毒性实验、热原实验、溶血...

Biocompatibility of silver-loaded coral hydroxyapatite.

Objective To evaluate the biocompatibility of silver-loated coral hydroxyapatite (Ag-CHA) at 8×10-5 mol/L Ag+ concentration. Methods Silver-loaded coral hydroxyapatite (Ag-CHA) at 8×10-5 mol/L Ag+ concentration was prepared by treating with AgNO3 and NaCl solution, followed by vacuum freeze-drying. To access the biocompatibility of Ag-CHA, the systemic acute toxicity test, pyrogen test, hemolysis test and dermal sensitization test were carried out on healthy adult Kunming mice, New Zealand rabbits, and albino guinea pigs. Results In systemic acute toxicity test, the mice did not lose their weight and had no toxic reaction in 72 hours, 8×10-5 mol/L Ag-CHA had no systemic acute toxicity. In pyrogen test, the highest temperature increments of rabbits were 0.2, 0.2 and 0.4℃ respectively. In hemolysis test, the hemolysis rate was 0.64%. In dermal sensitization test, the skin of guinea pigs in experimental group had no erythema and hydroderma. Conclusion Ag-CHA with 8×10-5 mol/L Ag+ concentration had good biocompatibility and had no systemic acute toxicity, no pyrogen, no hematolysis and no allergen.