口腔中幽门螺杆菌与RAU的关系研究

目的:检测复发性阿弗他溃疡(RAU)患者口腔中幽门螺杆菌(H.pylori),探讨H.pylori感染与RAU的关系.方法:采用巢式PCR方法,利用内引物(ET-5U/ET-5L)、外引物(EHC-U/EHC-L),检测42例RAU患者口腔溃疡表面的假膜和上皮组织刮取物以及患者口腔其他部位正常黏膜上皮刮取物.结果:RAU患者溃疡组织、患者正常完整黏膜中H.pylori阳性率分别为16.7%(7/42)、7.1%(3/42),两者之间无显著性差异(P＞0.05);并且RAU患者溃疡组织与对照组正常完整口腔黏膜中H.pylori阳性率4.8%(2/42),两者之间也无显著性差异(P＞0.05).结论:H.pylori和RAU的发病可能无直接相关性.

Research on the relationship between helicobacter pylori and recurrent aphthous ulcer

Objective:To study the possible role of Helicobacter pylori(H.pylori) in the pathogenesis of recurrent aphthous ulcer(RAU),we detected the prevalence of H.pylori in the oral cavity of the patients with RAU.Method:The nested polymerase chain reaction(N-PCR) was used to detect the presence of H.pylori in the oral cavity of the patients with RAU and the control group.The amplification was performed with outer primers(EHC-U/EHC-L) and inner primers(ET-5U/ ET-5L).Result:7 RAU lesions(16.7%) were PCR positive for H.pylori,while 3 cases(7.1%) were positive in the contralateral intact mucosa of RAU patients.No statistically significant relation was found between the presence of H.pylori and the occurrence of RAU(P>0.05).2 cases(4.8%) were positive in the intact oral mucosa of the control group by N-PCR.The difference of the frequency of H.pylori between RAU lesion in the case group and intact oral mucosa in the control group was not statistically significant(P>0.05).Conclusion:The present study does not give support to the assumption that H.pylori could be involved in the pathogenesis of RAU.