慢性肝损伤致肝纤维化过程中微小RNA200家族的表达

目的观察肝组织中微小RNA200(miR-200)家族成员在慢性肝损伤致肝纤维化过程中表达变化并探讨其机制。方法雄性Wistar大鼠皮下注射四氯化碳(CCl4)制备肝纤维化模型,分别在2、4、6、8周处死大鼠测定肝脏指数、血清ALT、AST含量,观察肝组织病理改变,Real-time PCR检测肝组织miR-200a、b、c、141、429mRNA表达变化。结果模型各组大鼠肝脏指数、血清ALT、AST含量显著高于正常对照组(P<0.01),8周模型组肝纤维化明显,8周模型组肝组织中miR-200a、b、c、141、429mRNA表达较正常组显著增加(P<0.05)。结论 miR-200s在慢性肝损伤致肝纤维化过程中的表达变化,提示其参与慢性肝损伤及肝纤维化的发生发展。

The dynamic changes of miR-200s in rat liver during the development of chronic liver injury induced liver fibrosis

Objective To observe the dynamic changes of Micro-RNA 200(miR-200s) Family in rat liver during the development of chronic liver injury induced liver fibrosis.Methods Forty male Wistar rats weighing 180-220 g were divided into five groups(control group and four model groups).Model groups were induced by hypodermic injection of CCl4 for 2,4,6 and 8 weeks.The liver index and the content of ALT and AST in serum were analyzed,and the liver fibrosis were observed by microscope.The expression of miR-200a,miR-200b,miR-200c,miR-141 and miR-429 mRNA were determined by using Real-time PCR respectively.Results The liver index,ALT and AST in four model groups were obviously higher than normal control group.The apparent liver fibrosis was observed in 8 weeks model group.The expression of miR-200a,b,c,141 and 429 mRNA in rat liver of 8 weeks model groups were obviously higher than that in control.(P<0.05).Conclusion In the process of liver fibrosis induced by CCl4,the obvious change of miR-200s reveals that the miR-200s may play an important role in the chronic liver injury and liver fibrosis.