电针对创伤应激大鼠胸腺细胞凋亡的影响

目的:观察创伤应激及电针对大鼠胸腺细胞增殖功能及细胞凋亡的影响。方法:Sprague-Dawley大鼠随机分为正常对照组、创伤应激组、创伤应激加电针组。建立创伤应激大鼠模型。电针选用的穴位为“足三里”和“阑尾”两穴。用MTT法检测胸腺细胞增殖功能,用TdT介导的脱氧尿嘧啶末端缺口标记法(TUNEL)检测胸腺细胞凋亡情况。结果:创伤应激能导致大鼠胸腺细胞发生凋亡,同时伴有胸腺细胞增殖能力的降低;电针能减少由于创伤应激诱导的胸腺细胞凋亡并改善胸腺增殖能力。结论:电针可通过抑制创伤应激诱导的胸腺细胞凋亡,起到改善应激后细胞免疫功能紊乱的作用。

Effect of Electroacupuncture on Thymocyte Proliferation and Apoptosis in Surgical Trauma-stress Rats

Objective: To observe the effect of electroacupuncture(EA) on thymocyte proliferation and apoptosis in surgical trauma-stress rats.Methods: Thirty-six SD rats were evenly randomized into control,model and EA groups.Surgical trauma-stress model was established by making two longitudinal incisions((6 cm) and(5 cm) long) along the median back and abdominal lines and performing visceral exploration manipulation.EA((2 Hz) and(60 Hz) in the frequency,(2.85 s)/(1.05 s) in the duration of pulse-trains,and(1 mA)) was applied to unilateral "Zusanli"(ST 36) and "Lanwei"(EX-LE 7) for(30 min).After killing the rats,the thymus tissue was sampled to be processed into cell suspension for determining the thymocyte proliferation which was expressed by using stimulus index [optical density(OD) of the cellular culture fluid of EA group/OD of control group],and thymocyte apoptosis was assayed by TdT-mediated dUTP nick end labeling(TUNEL) technique.Results: Results showed that the rates of the apoptotic thymocytes in normal control,model and EA groups were 3.56±3.18%,15.32±4.67%,and 7.42±4.19% respectively;while the SI values of the aforementioned 3 groups were 1.96±0.09,1.40±0.10 and 1.65±0.10 separately.It indicated that after trauma-stress,the apoptosis of the thymus increased markedly and the cellular proliferation lowered significantly(P<0.01,0.001);following EA,the aforementioned effects of surgical trauma-stress were suppressed apparently(P<0.05).Conclusion: EA can improve trauma-stress induced immunodysfunction via inhibiting the apoptosis and facilitating the proliferation of thymocytes.