| 酶联免疫吸附检测重症肌无力患者血清中抗乙酰胆碱受体配体抗体的独特型抗体 |
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| 引用本文： | 黄德仁,涂来慧,张仁琴,周广智,沈茜. 酶联免疫吸附检测重症肌无力患者血清中抗乙酰胆碱受体配体抗体的独特型抗体[J]. 第二军医大学学报, 1990, 0(2) |
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| 作者姓名： | 黄德仁 涂来慧 张仁琴 周广智 沈茜 |
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| 作者单位： | 长海医院神经内科(黄德仁，涂来慧，张仁琴，周广智)，长海医院检验科(沈茜) |
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| 摘 要： | 用具有内影像的nAchR配体抗体——银环蛇毒抗血清为工具,采用酶联免疫吸附法(ELISA)检测了81例重症肌无力(MG)患者血清中特异性相应的抗独特型抗体(Anti-Id),其中阳性38例,阳性率46.9％,用nAchR进行的ELISA特异性交叉抑制试验阳性。对各改良Osserman分型MG及MG是否伴胸腺瘤病人血清进行了Anti-Id ELISA检测,并与放射免疫沉淀法测定nAchR-Ab进行了比较,并对nAchR-配体抗体的Anti-Id在MG发病中的作用及Anti-Id的治疗前景作了探讨。
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| 关 键 词： | 重症肌无力 酶联免疫吸附法 烟碱型乙酰胆碱受体 配体 银环蛇毒抗血清 独特型抗体 |
ELISA for Detection of Anti-idiotype Antibodies to Antibodies to Ligand of Nicotinic Acetylcholine Receptor in Sera of Patients with Myasthenia Gravis |
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| Huang Deren,et al. ELISA for Detection of Anti-idiotype Antibodies to Antibodies to Ligand of Nicotinic Acetylcholine Receptor in Sera of Patients with Myasthenia Gravis[J]. Former Academic Journal of Second Military Medical University, 1990, 0(2) |
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| Authors: | Huang Deren et al |
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| Abstract: | Anti-bungarotoxin anti-serum, which has the internal image of nicotinic acetylcholine receptor (nAchR), was used as a tool to measure anti-idiotype antibodies(Anti-Id) to antibodies to ligand of nAchR in sera from 81 with myasthenia gravis (MG). Enzyme-linked immunosorbent assay (ELISA) was adopted. The positive rate was 46. 9% (38/81). The specific cross inhibitory test with nAchR was positive. Anti-Id to antibodies to ligand of nAchR in sera of different types of MG patients classified according to modified Osserman's standards and MG patients with or without thymoma were compared in this study and the role of Anti-Id to antibodies to ligand of nAchR in the autoimmunity of MG and the possibility of therapeutic use of Anti-Id are discussed. |
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| Keywords: | myasthenia gravis enzyme-linked immunosorbent assay nicotinic acetylcholine receptor ligand anti-bungarotoxin anti-serum antHdiotype antibody |
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