丹参及β-七叶皂甙钠对烫伤大鼠急性肺损伤的抑制作用

目的 探讨丹参及β-七叶皂甙钠对烫伤大鼠急性肺损伤(acute lung injury，ALI)的治疗作用及其机制，为临床治疗烧伤后ALI提供理论依据。方法 制作30％TBSAⅢ度大鼠烫伤模型，并随机分为盐水组、丹参组、β-七叶皂甙钠组及联合组，另设假烫对照组，每组9只。烫伤后24h取静脉血测外周血白细胞粘附聚集(LAA)；取肺组织测髓过氧化物酶(MPO)、丙二醛(MDA)、超氧化物歧化酶(SOD)含量及肺组织湿干重量比(W／D)。结果 丹参组、β-七叶皂甙钠组及联合组外周血LAA、肺组织MPO及MDA、肺组织W／D明显低于盐水组，联合组最低；SOD含量明显高于盐水组，联合组最高。外周血LAA与肺组织MPO含量呈正相关。结论 丹参及β-七叶皂甙钠可减轻中性粒细胞(PMN)在肺内的聚集、粘附，减轻氧自由基(OFR)及其代谢产物对肺组织的损伤，增加抗氧化剂含量，降低肺微血管通透性和肺水含量，对烧伤后肺损伤有一定防治作用，联合应用效果更佳。

Inhibitory effects of salviae miltrorrhizae and β-aescinom natricum on postburn acute lung injury in rats

OBJECTIVE: To explore the inhibitory effect and its mechanism of salviae miltrorrhizae and beta-aescinom natricum on the postburn acute lung injury in rats. METHODS: Forty-five rats were randomly divided into sham control (C, n = 9), sodium chloride group (S, n = 9), salviae miltrorrhizae group (M, n = 9), beta-aescinom natricum group (A, n = 9), and combination group (MA, n = 9). The rats in M, A and MA groups were subjected to 30% TBSA III degree scald on the back, and all the rats were sacrificed at 24 PBH. The blood and pulmonary tissue samples were harvested from the rats at 24 PBH for the determination of leukocyte adhesiveness/aggregation (LAA) in peripheral blood, myeloperoxidase (MPO), malondialdehyde (MDA) and superoxide dismutase (SOD) contents, and the ratio of wet to dry weights (W/D) of lung tissue. RESULTS: Compared with those in S group, the LAA in blood and the pulmonary tissue contents of MPO, MDA and W/D rate in M and A groups, and especially in MA group, were decreased significantly, but the SOD content in pulmonary tissue increased obviously in M and A groups, especially in MA group. Furthermore, blood LAA was positively correlated with pulmonary tissue MDA content. CONCLUSION: Postburn intra-pulmonary agglutination and aggregation of PMNs and pulmonary injury by oxygen free radicals (OFRs) and their products could be inhibited by either Salviae Miltrorrhizae or beta-aescinom natricum. In addition, these agents could also increase the tissue content of antioxidant capacity and decrease pulmonary microvascular permeability and lung water content. The results indicated that all the agents used might be effective in prevention and treatment of postburn pulmonary injury, especially when used together.