| Chemical profiles and anticancer effects of saponin fractions of different polarity from the leaves of Panax notoginseng简 |
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| 引用本文： | MAO Qian,LI Yi,LI Song-Lin,YANG Jie,ZHANG Ping-Hu,WANG Qiang. Chemical profiles and anticancer effects of saponin fractions of different polarity from the leaves of Panax notoginseng简[J]. 中国天然药物, 2014, 0(1): 30-37 |
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| 作者姓名： | MAO Qian LI Yi LI Song-Lin YANG Jie ZHANG Ping-Hu WANG Qiang |
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| 基金项目： | This project was supported by the National Natural Science Foundation of China （Nos. 81274018, 81274068） and the Project funded by Jiangsu Branch of China Academy of Chinese Medical Science （JSBY 1306）. |
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| 摘 要： | AIM： To evaluate the chemical profiles and cytotoxic effects among the total saponin fraction （TSF）, 25% ethanol fraction （25EF）, 50% ethanol fraction （50EF）, and 85% ethanol fraction （85EF） prepared by macroporous resin from the leaves of Panax notoginseng. METHOD： The simultaneous determination of thirteen main saponins, as well as the chemical profiles of saponin fractions of different polarity, was made by HPLC-DAD and LC-ESI-MSn analysis. The cytotoxic effects were determined against KP4 cells （human pancreatic cancer）, NCI-H727 ceils （human lung cancer）, HepG2 cells （human hepatocellular cancer）, and SGC-7901 cells （human gastric adenocarcinoma）. RESULTS： Chemical analysis indicated that 85EF possessed the most abundant cytotoxic protopanaxadiol saponins, including the marker saponins F2, 20（R）-Rg3, 20（S）-Rg3, and Rh2. The MTT assay showed that 85EF also had the strongest cytotoxic effects among the four fractions. 25EF showed no anti-proliferative effects, while 50EF and TSF exhibited weak anti-proliferative activity. CONCLUSION： From the aspect of comprehensive utilization of resources, 85EF, enriched with low polarity PPD group saponins, is a new alternative source of anticancer saponins, and a promising botanical preparation for further anticancer studies.
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| 关 键 词： | 中药学 中药材 植物药 药理分析 |
Chemical profiles and anticancer effects of saponin fractions of different polarity from the leaves of Panax notoginseng |
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| MAO Qian,LI Yi,LI Song-Lin,YANG Jie,ZHANG Ping-Hu,WANG Qiang. Chemical profiles and anticancer effects of saponin fractions of different polarity from the leaves of Panax notoginseng[J]. Chinese JOurnal of Natural Medicines, 2014, 0(1): 30-37 |
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| Authors: | MAO Qian LI Yi LI Song-Lin YANG Jie ZHANG Ping-Hu WANG Qiang |
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| Affiliation: | [1]Department of Pharmaceutical Analysts and Metabolomics, Jiangsu Branch of China Academy of Chinese Medical Science,Nanjing 210028, China [2]State Laboratory of Modern Chinese Medicines, China Pharmaceutical University, Nanjing 210009, China [3]Nanjing Normal University Center for Analysis and Testing, Nanjing Normal University, Nanjing 210009, China [4]Jiangsu Center for New Drug Screening ＆ National New Drug Screening Laboratory, China Pharmaceutical University,Nanjing 210009, China |
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| Abstract: | Panax notoginseng Leaf material Saponin Chemical profile Cytotoxic effects |
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| Keywords: | Panax notoginseng Leaf material Saponin Chemical profile Cytotoxic effects |
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