| 长白白眉蝮蛇类凝血酶的研究Ⅰ：纯化方法的研究 |
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| 引用本文： | 王晓军,何宝俊.长白白眉蝮蛇类凝血酶的研究Ⅰ：纯化方法的研究[J].中国医科大学学报,1991,20(2):114-120. |
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| 作者姓名： | 王晓军 何宝俊 |
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| 作者单位： | 中国医科大学医学基础部化学检测中心实验室
(王晓辉)，中国医科大学医学基础部化学检测中心实验室(何宝俊) |
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| 摘 要： | 采用四种纯化方法,对长白白眉蝮蛇类凝血酶的纯化途径进行研究。结果,阴阳离子交换柱、分子筛柱层析法周期较长,收率较低。羟基磷灰石柱法。对类凝血酶有较好的分离效果,但要求样品量小且纯度较高。亲和层析法分离类凝血酶,具有操作简便、纯化周期短、回收率高、处理样品量大等特点。
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| 关 键 词： | 蛇毒 类凝血酶 纯化 |
STUDY ON PURIFICATION OF A THROXBN-LIKE E ZYME FROM VENOM OF AGKISTRODON HALYS USSURIENSIS ENELIANOV |
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| Wang Xiaohui,et al Laboratory center of China Medical University.STUDY ON PURIFICATION OF A THROXBN-LIKE E ZYME FROM VENOM OF AGKISTRODON HALYS USSURIENSIS ENELIANOV[J].Journal of China Medical University,1991,20(2):114-120. |
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| Authors: | Wang Xiaohui Laboratory center of China Medical University |
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| Abstract: | A thrombin-like enzyme has been purified homogeneitically by SDS-PAGEfrom the venom of Agkistrodon halys ussuriensis Emelianov by mean of home-made affinity column on fast protein liquid chromatography system. Thismethod is rapider and more convenient and high over-all recovery than bymeans of ion-exchange chromatography and gel filtration chromatography. |
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| Keywords: | snake venom thrombin-like enzyme lurification |
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