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| 银纳米颗粒对神经元的毒性研究 | |
| 引用本文： | 周国凤,汤京龙,熊玲,周亮,许建霞,邵安良,杨丽娜,陈艳梅,李雪飞,奚廷斐. 银纳米颗粒对神经元的毒性研究[J]. 北京生物医学工程, 2011, 30(3): 301-305. DOI: 10.3969/j.issn.1002-3208.2011.03.17 |
| 作者姓名： | 周国凤 汤京龙 熊玲 周亮 许建霞 邵安良 杨丽娜 陈艳梅 李雪飞 奚廷斐 |
| 作者单位： | 1. 温州医学院,浙江,温州,325035;中国药品生物制品检定所,北京,100050 2. 中国药品生物制品检定所,北京,100050 3. 成都市食品与药品检测中心,成都,610041 4. 温州医学院,浙江,温州,325035 5. 温州医学院,浙江,温州,325035;北京大学深圳研究院,广东深圳,510087 6. 温州医学院,浙江,温州,325035;中国药品生物制品检定所,北京,100050;北京大学深圳研究院,广东深圳,510087 |
| 基金项目： | 国家自然科学基金，973项目，863项目，中国药品生物制品检定所中青年研究发展基金 |
| 摘 要： | 目的 研究不同浓度的银纳米颗粒对神经元毒性的剂量-效应关系,探索银纳米颗粒对神经元的毒性机理.方法 首先培养一种活性好、生长状态优良的原代神经元,将不同浓度（2.5～500μg/mL）的银纳米颗粒加入神经元中作用24h后,通过MTT法计算细胞增殖率,并分析不同浓度的银纳米颗粒对神经元毒性的剂量-效应关系.结果 在25～250μg/mL范围内,细胞数量与形态呈现不同的变化,银纳米颗粒浓度与细胞增殖率呈负相关.结论 在25～250μg/mL的浓度范围内,银纳米颗粒对神经元细胞的毒性呈剂量-效应关系. |
| 关 键 词： | 银纳米颗粒 神经元 毒性 |
Toxicity of Silver Nanoparticles to the Neurons |
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| ZHOU Guofeng,TANG Jinglong,XONG Ling,ZHOU Liang,XU Jianxia,SHAO Anliang,YANG Lina,CHEN Yanmei,LI Xuefei,XI Tingfei. Toxicity of Silver Nanoparticles to the Neurons[J]. Beijing Biomedical Engineering, 2011, 30(3): 301-305. DOI: 10.3969/j.issn.1002-3208.2011.03.17 | |
| Authors: | ZHOU Guofeng TANG Jinglong XONG Ling ZHOU Liang XU Jianxia SHAO Anliang YANG Lina CHEN Yanmei LI Xuefei XI Tingfei |
| Affiliation: | 1 Wenzhou Medical College, Wenzhou, Zhejiang Province 325035 2 National Institute for the Control of Pharmaceutical and Biological Products, Beijing 100050 3 Food and Drug Testing Center in Chengdu,Chengdu 610041 4 Shenzhen Institute of Peking University, Shenzhen, Grangdong Province 510087 |
| Abstract: | Objective To study the dose-effect relationship between silver nanoparticles with different concentrations and the neurotoxicity, and also explore the mechanism of silver nanoparticles to neurotoxicity. Method A group of primary neurons in excellent growth status was cultured, and then contacted with different concentrations （2.5 -500μg/ml ）of silver nanoparticles for 24h. After that we calculated the cell proliferation rate by MTT test and analyzed the dose-effect relationship between the concentrations of silver nanoparticle with neurotoxicity. Result Within the silver nanoparticle concenrtation of 25 -250μg/ml, both cellular mount and morphology changed, and it was showed anticorrelation between silver nanoparticle concentration and cell proliferation rate. Conclusion Within the sliver nanoparticles concenrtation of 25 -250μg/ml, silver nanoparticles shows a dose-effect relationship on neurotoxicity. |
| Keywords: | silver nanoparticle neuron toxicity |
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