端粒酶反义寡聚脱氧核苷酸诱导人胃癌细胞凋亡过程加端粒酶活性的测定

目的：观察端粒酶反义寡聚脱氧核苷酸对人胃癌细胞株的作用。方法：用端粒酶反义寡聚脱氧核苷酸与人胃癌细胞株共同孵育一定时间后，观察细胞形态变化，观察细胞DNA含量的分布，测定端粒酶活性。结果：端粒酶反义寡聚脱氧核苷酸能诱导人胃癌细胞凋亡，抑制端粒酶活性。在形态学上表现为细胞膜起泡、染色质固缩、核碎裂、凋亡小体形成。电冰呈凋亡特征性Ladder带；流式细胞仪分析显示，在G1期前出现亚2倍体凋亡峰；端粒酶活性抑制。结论：端粒酶反义寡聚脱氧核苷酸能诱导胃癌细胞凋亡，抑制端粒酶活性，抑制端粒形成，从而抑制胃癌细胞的生长。

The Effect of TelomeraseAntisense Oligodeoxynucleotides on the Apoptosis of Gastric CancerCell Line

To observe the effect of telomerase antisense oligodexynucleotides on gastric cancer cell line. Methods: Gastric cancer cell line were cultured with 1～5 μmol/L telomerase antisense oligodexynucleotides for various duration, morphologic changes and cell-DNA distribution were studied. The apoptotic cells were measured with flow cytometer. Telomerase activity were detected by TRAPELISA. Results: Telomerase antisense oligodeoxynucleotides acted as a specific growth inhibitor to gastric cancer cell line. Gastric cancer cells cultured with 5 μmol/L telomerase antisense oligodexynucleotides presented apoptotic features: inacecell membrane, chromatin condensation, nucleic fragmentation and apoptotic body formation; flow cytometry analysis showed a sub-Gl cell peak; agarose electrophoresis showed marked DNA ladder. Telomerase activity were inhibited. Conclusions: Telomerase may play a crucial role in the growth of gastric cancer cells. Telomerase antisense oligodeoxynucleotides may inhibit telomerase activity. It may be a new target of treatment to gastric cancer.