螺旋霉素与乙酰螺旋霉素的高效液相色谱定量分析

为了分离螺旋霉素和乙酰螺旋霉素,本文较系统地研究了正、反相高效液相色谱法。用μ-Bondapak C₁₈柱,以甲醇-0.01 mol/L H₃PO₄(45:55)为流动相,可将乙酰螺旋霉素各组分完全分离,以甲醇-0.01 mol/L H₃PO₄(40:60)为流动相,可将螺旋霉素三组分较好地分离。用Zorbax CN柱,以甲醇—异丙醇—环己烷为流动相,可将螺旋霉素三组分满意地分离。

QUANTITATIVE ANALYSES OF SPIRAMYCIN AND ACETYLSPIRAMYCIN BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

Normal and reversed-phase chromatographic methods have been systematically investigated for separating spiramycin(SPM) and acetylspiramycin (Ac-SPM). The four components of Ac-SPM have been well separated by HPLC using μ-Bondapak C₁₈ column with a mobile phase composed of 0.01 mol/L H₃PO₄-CH₃OH (55:45). Two chromatographic systems were chosen to estimate the three components of SPM. In the first system 0.01 mol/L H₃PO₄—CH₃OH (60:40) eluent is used, and in the second system Zorbax CN column with methanol—isopropyl alcohol—cyclohexane (5:6:89) mobile phase is employed. It has been found that the contents of each component in the SPM made in China are different from those made in Japan, namely, in the Chinese SPM, SPM Ⅱ and Ⅲ are the major components, but in Japanese SPM, SPM Ⅰ is the chief component. Moreover, in the Chinese Ac-SPM, diacetyl spiramycin Ⅱ, Ⅲ are dominant, while in the Japanese Ac-SPM, di-and monoacetyl spiramycin Ⅱ are the major components.