Alcoholic and isocaloric diet,but not ovariectomy,influence the apoptosis of bone cells within the alveolar bone crest of rats |
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| Affiliation: | 1. Department of Biosciences and Diagnostics, Institute of Science and Technology, UNESP – State University of Sao Paulo, São José dos Campos, São Paulo, Brazil;2. Restorative Dentistry Department, Institute of Science and Technology, UNESP – Univ Estadual Paulista, São José dos Campos, São Paulo, Brazil;3. Department of Preventive and Community Dentistry, College of Dentistry, University of Iowa, Iowa City, IA, USA;1. Division of Nephrology and Hypertension, Department of Medicine, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA;2. Department of Biochemistry and Molecular Biology, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA;3. Mayo Graduate School, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA;4. Department of Orthopedic Surgery, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA;1. Dental Department, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou 310016, China;2. Department of Oral and Maxillofacial Surgery, Stomatology Hospital, School of Medicine, Zhejiang University, Hangzhou 310006, China;3. Department of Oral Medicine, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China;1. Division of Nephrology and Hypertension, Mayo Clinic, 200 1st St., SW, Rochester, MN 55905, USA;2. Department of Internal Medicine, Mayo Clinic, 200 1st St., SW, Rochester, MN 55905 USA;3. Department of Cellular Biology and Anatomy, Georgia Regents University, Augusta, GA, USA;4. Department of Orthopedic Surgery, Mayo Clinic, 200 1st St., SW, Rochester, MN 55905, USA;5. Department of Biochemistry and Molecular Biology, Mayo Clinic, 200 1st St., SW, Rochester, MN 55905, USA;3. Musculoskeletal Disease Area and Novartis Institutes for BioMedical Research, 4056 Basel, Switzerland;4. Developmental and Molecular Pathways, Novartis Institutes for BioMedical Research, 4056 Basel, Switzerland;3. From the Center for Translational Medicine, Departments of Medicine, Sidney Kimmel Medical College, Thomas Jefferson University, Philadelphia, Pennsylvania 19107;6. Department of Orthopaedic Surgery, Sidney Kimmel Medical College, Thomas Jefferson University, Philadelphia, Pennsylvania 19107;4. the Department of Veterans Affairs Medical Center, Pittsburgh, Pennsylvania 15240, and;5. Departments of Pathology and of Cell Biology, School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania 15261 |
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| Abstract: | ObjectiveStudies suggest that chronic alcoholism as well as oestrogen deficiencies may affect bones in general, including alveolar bone and, by doing so, increase individuals’ susceptibility to develop progressive periodontal disease. This paper aims to verify the influence of chronic alcoholism and/or oestrogen deficiencies in the apoptosis of bone cells of the alveolar bone crest region in rats.DesignInitially, 54 rats were divided into ovariectomized (Ovx) and Sham operated (Sham) groups. Thirty days after surgery, these two groups were equally sub-divided, and received, for 56 days, the following dietary intervention: alcoholic diet (with 20% alcohol solution,), isocaloric diet and ad libitum diet (free diet). Analysis was undertaken by immunohistochemistry, using an antibody to detect apoptosis (anti PARP p-85).ResultsWhen comparing the six experimental groups, no significant differences were observed in the apoptosis of bone cells. Also, there was no significant difference in the quantity of cells undergoing apoptosis when the animals from Ovx groups were compared with those from Sham groups. However, when comparing only different dietary groups, differences were observed between the groups ad libitum and isocaloric, to osteoblasts (p = 0.045); and ad libitum and alcohol, to osteocytes (p = 0.007).ConclusionIt is concluded that ovariectomy was not able to influence the rate of apoptosis of bone cells of the alveolar bone crest region in rats and that a possible influence of diet on apoptosis of osteoblasts and osteocytes cannot be ruled out. |
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| Keywords: | Alcoholism Ovariectomy Osteoporosis Periodontium Apoptosis |
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