Control of microorganisms of oral health interest with Arctium lappa L. (burdock) extract non-cytotoxic to cell culture of macrophages (RAW 264.7) |
| |
| Institution: | 1. State Key Laboratory of Food Science and Technology, School of Food Science and Technology;2. National Engineering Research Center for Functional Food, Jiangnan University, Wuxi 214122, People''s Republic of China;3. Department of Food Science and Technology, University of California, Davis, California 95616, USA;1. Special Food Chemistry and Food Production, TU Dresden, Bergstraße 66, 01069 Dresden, Germany;2. Clinic of Operative Dentistry, University Hospital Carl Gustav Carus, Fetscherstraße 74, 01307 Dresden, Germany |
| |
| Abstract: | ObjectivesTo evaluate the antimicrobial activity of Arctium lappa L. extract on Staphylococcus aureus, S. epidermidis, Streptococcus mutans, Candida albicans, C. tropicalis and C. glabrata. In addition, the cytotoxicity of this extract was analyzed on macrophages (RAW 264.7).DesignBy broth microdilution method, different concentrations of the extract (250–0.4 mg/mL) were used in order to determine the minimum microbicidal concentration (MMC) in planktonic cultures and the most effective concentration was used on biofilms on discs made of acrylic resin. The cytotoxicity A. lappa L. extract MMC was evaluated on RAW 264.7 by MTT assay and the quantification of IL-1β and TNF-α by ELISA.ResultsThe most effective concentration was 250 mg/mL and also promoted significant reduction (log10) in the biofilms of S. aureus (0.438 ± 0.269), S. epidermidis (0.377 ± 0.298), S. mutans (0.244 ± 0.161) and C. albicans (0.746 ± 0.209). Cell viability was similar to 100%. The production of IL-1β was similar to the control group (p > 0.05) and there was inhibition of TNF-α (p < 0.01).ConclusionsA. lappa L. extract was microbicidal for all the evaluated strains in planktonic cultures, microbiostatic for biofilms and not cytotoxic to the macrophages. |
| |
| Keywords: | Biofilms RAW 264 7 |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
