Effects of heat-inactivated Bifidobacterium BB12 on cariogenicity of Streptococcus mutans in vitro |
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| Affiliation: | 1. Department of Operative and Preventive Dentistry, Charité – Universitätsmedizin Berlin, Aßmannshauser Str. 4-6, 14199 Berlin, Germany;2. Clinic for Conservative Dentistry and Periodontology, Christian-Albrechts-University, Arnold-Heller-Str. 4-6, 24105 Kiel, Germany;3. Centre of Dentistry, Biological Laboratory, Friedrich-Schiller-University, Bachstr. 18, 07740 Jena, Germany;1. Cereal Group, Instituto de Agroquímica y Tecnología de Alimentos (IATA-CSIC), Valencia, Spain;2. Lactic Acid bacteria and Probiotics Laboratory, Instituto de Agroquímica y Tecnología de Alimentos (IATA-CSIC), Valencia, Spain;1. Department of Pharmacy, Gansu Provincial Hospital, Lanzhou, Gansu, People’s Republic of China;2. Department of Medical Oncology, Gansu Gem Flower hospital, Lanzhou, Gansu, People’s Republic of China;1. Department of Translational Research, New Technologies in Medicine Surgery, University of Pisa, Italy;2. Department of Biomolecular Sciences, Division of Toxicological, Hygiene and Environmental Sciences, University of Urbino “Carlo Bo”, via S. Chiara 27, 61029 Urbino, PU, Italy;1. Center for One Health, College of Veterinary Medicine, Konkuk University, Seoul, South Korea;2. Department of Food and Nutrition, Hanyang University, Seoul, South Korea;3. USDA, ARS, Albany, CA, USA |
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| Abstract: | ObjectiveSince some probiotic bacteria are cariogenic themselves, their suitability for caries management is questionable. Inactivated bacteria or their supernatants have been found to exert probiotic effects, whilst having several advantages compared with living bacteria. We hypothesized that viable and heat-inactivated Bifidobacterium animalis BB12 reduces the cariogenicity of Streptococcus mutans (SM) in vitro.DesignWe assessed mono- and mixed species biofilms of SM and viable or heat-inactivated BB12. Biofilms were grown in a continuous-culture-system under cariogenic conditions on smooth proximal enamel or cavitated dentine. For each of eight experimental subsets (4 biofilms × 2 hard-tissue conditions), a total of 32 specimens was used. After 10 days, bacterial numbers of 12 biofilms per group were analysed, and all specimens submitted to transversal microradiography.ResultsMineral loss was higher in cavitated dentine than smooth enamel for all biofilms (p < 0.001, t-test). BB12-monospecies biofilms induced significantly less mineral loss than SM in both enamel (p < 0.05) and dentine (p < 0.001). Viable BB12 did not significantly reduce cariogenicity of SM (p > 0.05), whilst heat-inactivated BB12 decreased cariogenicity of SM in dentinal cavities (p < 0.01). Bacterial numbers were higher on dentine than enamel (p < 0.05), but not significantly influenced by biofilm species (p > 0.05).ConclusionsHeat-inactivated BB12 reduced the cariogenicity of SM in dentinal cavities in vitro. Inactivated probiotics might be suitable for caries control. |
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| Keywords: | Bacteria Biofilm(s) Caries Microbial ecology Plaque/plaque biofilms Probiotics |
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