Effect of generation 4.0 polyamidoamine dendrimer on the mineralization of demineralized dentinal tubules in vitro |
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| Affiliation: | 1. Department of Prosthodontics, Stomatological Hospital, College of Stomatology, Xi’an Jiaotong University, Xi’an, Shaanxi, China;2. Department of Physiology and Pathophysiology, Xi’an Jiaotong University Health Science Center, Xi’an, Shaanxi, China;3. Department of Prosthodontics, Xiamen Stomatological Hospital, Xiamen, Fujian, China;1. Department of Pediatric Dentistry, Piracicaba Dental School, University of Campinas, Piracicaba, São Paulo, Brazil;2. Department of Restorative Dentistry, University of Campinas, Piracicaba, São Paulo, Brazil;3. Department of Oral Biology, University of Leeds, St James University Hospital, Leeds UK;1. Graduate School at Shenzhen, Tsinghua University, Shenzhen, China;2. Department of Computer Science, The University of Central Arkansas, Conway, USA;3. Schools of Software, University of Technology, Sydney, Australia;1. Department of Preventive and Restorative Dental Sciences, University of California, San Francisco, 707 Parnassus Avenue, San Francisco, CA, 94143-0758, USA;2. Department of Materials Science and Engineering, University of Florida, Gainesville, FL 32611-6400, USA;1. State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, Department of Cariology and Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China;2. Department of Advanced Oral Sciences and Therapeutics, University of Maryland School of Dentistry, Baltimore, MD 21201, USA;3. Department of Prosthodontics, School of Stomatology, Shandong University, Shandong Provincial Key Laboratory of Oral Tissue Regeneration, Jinan, 250012, China;4. Center for Stem Cell Biology & Regenerative Medicine, University of Maryland School of Medicine, Baltimore, MD 21201, USA;5. Department of Mechanical Engineering, University of Maryland Baltimore County, Baltimore County, MD 21250, USA |
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| Abstract: | ObjectiveDentine hypersensitivity is a type of clinical oral disease, which is highly prevalent worldwide. Although there are many materials to treat dentine hypersensitivity, their long-term therapeutic effects are not satisfactory. Therefore, the aim of this research was to observe and identify the biological mineralization of the generation 4.0 polyamidoamine dendrimer on the demineralized dentinal tubules at different time points.Design2 mm-thick slices were obtained from the cemento-enamel junction of 36 third molar teeth that simulated the condition of sensitivity with acid etching. Slices were treated with generation 4.0 polyamidoamine dendrimer and peptide bond condensing agent, while no treatment was applied on the slices of the control group. Following immersion in artificial saliva for 2, 4, 6, and 8 weeks respectively, the mineralization condition of dentine slices was observed using the scanning electron microscope (SEM). In addition, the differences in the samples of dental slices between the 2 groups were also detected using the microhardness test.ResultsSEM results showed that the average diameter and density of the dentinal tubules in the experimental group were significantly lower than those in the control group (P < 0.001). The microhardness test exhibited a similar result, which suggested that the microhardness of the experimental group was significantly higher than the control group (P < 0.001).ConclusionGeneration 4.0 polyamidoamine dendrimer promotes the biomineralization of demineralized dentinal tubules. Moreover, this result also suggests that the 4.0th generation polyamidoamine dendrimer has the potential value for dentine hypersensitivity treatment. |
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| Keywords: | Dentine sensitivity Tooth remineralization Amides Dendrimers |
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