三氧化二砷抑制人胆囊癌细胞生长及对细胞周期的影响

目的:观察三氧化二砷(As_2O_3)对人胆囊癌细胞体内外生长的抑制作用及对细胞周期的影响。方法:不同浓度As_2O_3作用于人胆囊癌GBC细胞,用MTT法检测细胞生长情况,流式细胞仪检测细胞周期分布;20只BALB/c裸鼠皮下接种GBC细胞,随机分为0.9%氯化钠(NS)组(n=10)和As_2O_3组(n=10)。腹腔注射0.9%氯化钠0.2ml·只~1(NS组)或As_2O_3 10 mg·kg~(-1)(As_2O_3组),每周2次,连续7周,测量肿瘤的体积。结果:As_2O_3能抑制体内、外GBC细胞生长.体外实验中As_2O_3作用呈时间、剂量效应关系;流式细胞仪检测发现As_2O_3可将增殖过程中的GBC细胞阻滞于G1期。结论:As_2O_3能明显抑制体内外人胆囊癌GBC细胞的生长及引起G1期阻滞。

Effect of Arsenic Trioxide on the Growth and Cell Cycle of Human Gallbladder Carcinoma Cell

Objective:To observe the inhibitory effect of As_2O_3 on the growth of human gallbladder carcinoma in vivo and in vitro and the alteration of cell cycle.Methods:Human gallbladder carcinoma cells(GBC)were cultured with different concentra- tions of As_2O_3,the proliferative activity of cells was detected by MTT methods.and the cell cycle status were carried out by flow cytometry(FCM).Twenty BALB/c nude mice,inoculated with the GBC cells subcutaneously,were randomly divided into nature saline(NS)and As_2O_3 groups.0.2ml NS or 10mg As_2O_3/kg was injected biweekly into the abdominal cavity of every mouse in.NS group or As_2O_3 group for seven weeks.The tumor size was measured.Results:As_2O_3 may inhibit the growth of human gallbladder carcinoma in vivo and in vitro,and in a time-and dose-dependent manner in vitro.GBC cells treated with As_2 O_3 were arrested in G1 phase.Conclusion:As_2O_3 can significantly inhibit the growth of human gallbladder carcinoma in vivo and in vitro as well as induce the G1 phase cell cycle arrest in vitro.