Measurement of cell impedance in frequency domain using discontinuous current clamp and white-noise-modulated current injection |
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Authors: | M. Weckström E. Kouvalainen M. Juusola |
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Affiliation: | (1) Department of Physiology, University of Oulu, Kajaanintie 52A, SF-90220 Oulu, Finland |
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Abstract: | A method is decribed for the determination of cellular input impedance of non-spiking neurones. The input impedance is important when cellular geometry and the effects of voltage-dependent channels are considered. Cells are impaled with a single glass microelectrod and current is injected using a time-sharing technique. The cell's impedance is measured by randomly modulating the injected current and calculating the impedance as a transfer function between current and recorded membrane voltage. Corresponding coherence functions can also be calculated for estimating the signal-to-noise ratio, and also linearity (i.e. possible activation of voltage-dependent conductances) of the membrane. |
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Keywords: | Non-Spiking Vision Photoreceptor Potassium channels FFT |
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