Heterogeneity in intergenic regions of the ribosomal repeat of the pine-blister rustsCronartium flaccidum andPeridermium pini |
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| Authors: | Salvatore Moricca Takao Kasuga Keith Mitchelson Alessandro Ragazzi Stephanos Diamandis |
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| Institution: | (1) Present address: CNR, Centro di Studio per la Patologia della Specie Legnose Montane, Piazzale delle Cascine 28, I-50144 Firenze, Italy;(2) Istituto di Patologia e Zoologia Forestale e Agraria, Università di Firenze, Piazzale delle Cascine 28, I-50144 Firenze, Italy;(3) Department of Molecular and Cell Biology, University of Aberdeen, AB9 1AS Aberdeen, UK;(4) Forest Research Institute, 57006 Vassilika, Thessaloniki, Greece;(5) ForBio Research Pty Ltd., 50 Meiers Road, 4068 Indooroopilly, Queensland, Australia |
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| Abstract: | Mixed aeciospore isolates ofCronartium flaccidum andPeridermium pini were obtained from single-tree infections in Britain, Italy and Greece. The 5.8s ribosomal RNA gene and flanking intergenic transcribed spacer regions ITS 1 and ITS2 were found to be highly similar betweenC. flaccidum andP. pini. Within samples heterogeneity was detected at three nucleotide loci in the ITS1 and at four loci in the ITS2 suggesting that several fungal genotypes may occur at a single infection court. The heterogeneity was confirmed by heteroduplex polymorphism analysis of mixed aeciospore products. RFLP of the ribosomal intergenic spacer region 1 (IGSI) amplified from the same templates indicated limited sequence polymorphism in some copies of this repeated locus. Both the sexual and asexual forms ofC. flaccidum show evidence of sequence polymorphism in two independent, non-coding regions of the ribosomal gene array. Variation appears to be greater in the sexual formC. flaccidum, than in the monoaecious formP. pini. |
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| Keywords: | Cronartium flaccidum Pine blister rusts Ribosomal intergenic region polymorphism RFLP HPA analysis |
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