头针对急性脑缺血再灌注大鼠的保护作用

目的:探讨头针对急性脑缺血损伤的保护作用及机制。方法:将90只健康SD雌性大鼠随机分为假手术组10只、模型组40只、头针组40只,采用线栓法制备大脑中动脉阻塞(MCAO)再灌注模型,假手术组不造成缺血状态,头针组行头针治疗,于再灌注6、24、487、2 h时采用神经功能缺损量表(NSS)评定各组大鼠神经功能、TUNEL法检测各组神经细胞凋亡率,Western blot和RT-PCR检测脑组织Caspase-3、Bcl-2、Bax的表达。结果:头针组再灌注72 h NSS评分显著低于模型组(P<0.01);模型组缺血侧神经细胞凋亡率随再灌注时间延长而增加,24 h达高峰;头针组神经细胞凋亡率明显低于模型组,以244、8 h尤为显著。再灌注6 h后Bcl-2、Bax蛋白在缺血侧脑组织中均有表达,24 h达高峰,但随着时间推移,头针组Bcl-2/Bax比值减少较模型组慢;头针组的Bcl-2、Caspase-3 mRNA的表达较模型组比较均有统计学差异(P<0.05)。结论:脑缺血可诱导Bcl-2、Bax、Caspase-3基因表达增强;头针对缺血脑组织具有保护作用,可能通过抑制细胞凋亡、减轻脑缺血再灌注损伤来实现。

Protection of Scalp-acupuncture on Rats with Acute Cerebral Ischemia-reperfusion Injury

Objective:To explore the protection and mechanism of scalp-acupuncture(SA) on acute cerebral ischemia.Methods:Ninety SD rats were divided randomly into group sham-operated,model and SA,and MCAO models were established.The sham-operated group was not resulted in ischemia,and the SA gruop was given SA therapy.At 6,24,48 and 72 h after reperfusion,neurological sevity score(NSS) was used to evaluate the neuralfunction of rats,and to detect apoptosis rate of neuron by TUNEL.To determine the expression of Caspase-3,Bcl-2 and Bax by RT-PCR and Western blot.Results:The NSS score of SA group decreased at 72 h compared with model group(P<0.01).The neuron apoptosis rate of model group increased with a few extension of time and reached a peak at 24 h.The neuron apoptosis rate of SA group was less than model group,especially at 24 h and 48 h(P<0.01).The expression of Bcl-2 and Bax in ischemia side of brain could reach a peak at 24 h,The mRNA of Bcl-2 and Caspase-3 were significantly different with the model group.Conclusion:Cerebral ischemia can induce high expresssion of Bcl-2,Bax,Caspase-3 gene.SA can protect ischemic brain tissue by inhibiting neuron apoptosis.